Predictive value of micronucleus count in cervical intraepithelial neoplasia and carcinoma
| Autor: | Manjiri D Phansalkar, Marie Moses Ambroise, Kanchana Balasundaram |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Pathology Binucleated cells Uterine Cervical Neoplasms Cervical intraepithelial neoplasia Gastroenterology Sensitivity and Specificity Pathology and Forensic Medicine Internal medicine Carcinoma medicine Vaginal smear Humans Micronuclei Chromosome-Defective Retrospective Studies Cervical cancer Vaginal Smears Micronucleus Tests business.industry medicine.disease Uterine Cervical Dysplasia Squamous intraepithelial lesion ROC Curve Micronucleus test Carcinoma Squamous Cell Female business Micronucleus Papanicolaou Test |
| Zdroj: | Turk patoloji dergisi. 29(3) |
| ISSN: | 1309-5730 |
| Popis: | Objective: A micronucleus is a small additional nucleus formed due to chromosomal loss or fragmentation. Th e aim of this study was to assess the utility of micronucleus count as a screening tool in cervical cancer. Material and Method: A total of 132 cases consisting of 42 pre-neoplastic and neoplastic cases and 90 non-neoplastic cases were studied. Only routine Papanicolaou-stained cervical smears were used. In each smear, the number of micronucleated cells and binucleated cells were counted under oil immersion and expressed as a count per 1,000 cells. Th e micronucleated and binucleated cell counts in pre-neoplastic, neoplastic and non-neoplastic categories were compared for statistical significance. Th e counts were also correlated with age, duration of marital life, number of pregnancies and abortions. Results: Th e micronucleus count and the binucleated cell count were significantly higher in the high -grade squamous intraepithelial lesion (HSIL) and invasive carcinoma categories compared to low -grade squamous intraepithelial lesion (LSIL) and non-neoplastic cases. Receiver operating characteristic (ROC) curve analysis revealed that micronucleus count and binucleated cell count possess a high degree of sensitivity and specificity for identifying HSIL and invasive carcinoma. Conclusion: Our results reveal that micronucleus evaluation in routine Pap smears is a very useful biomarker in cervical cancer screening. Th e binucleated cell count increases the predictive value of this simple test. |
| Databáze: | OpenAIRE |
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