CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene
| Autor: | Shou-Mei Zhang, Zhi-Bin Qiao, Lu Zhang, Han-Yu Zhu, Zhi-Hui Bai, Hong-Xia Cao, Ji-Zhen Lu, Wen-Wen Jia, Zhong-Min Liu, Yi-Yao Qi, Yan Bao |
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| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Induced Pluripotent Stem Cells Methyltransferases Cell Biology General Medicine Biology Epigenesis Genetic Cell biology Histones Repressor Proteins Histone H3 Exon Histone methyltransferase Histone Methyltransferases Humans CRISPR Epigenetics Biology (General) CRISPR-Cas Systems Induced pluripotent stem cell DOT1L Gene Developmental Biology SUV39H1 |
| Zdroj: | Stem Cell Research, Vol 56, Iss, Pp 102519-(2021) |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2021.102519 |
| Popis: | SUV39H1 is a histone methyltransferase involve numerous biological processes, including of aging, embryo development, tumor growth and mitosis via catalysis of dimethylation and trimethylation of lysine 9 of histone H3. Here we report a human induced pluripotent stem cell line (EHTJUi005-A-1) which is generated from a wildtype human iPSC previously established in our laboratory, and this iPSC has a homozygous knockout of 8 bp in Exon 2 of SUV39H1. This iPSC model provides a valuable resource to study epigenetic regulation in extensive biological processes as mentioned above. |
| Databáze: | OpenAIRE |
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