Influence of bifentrin, a pyrethriod pesticide, on human colorectal HCT-116 cells attributed to alterations in oxidative stress involving mitochondrial apoptotic processes
| Autor: | Amal Salah, Intidhar Ben Salem, Chayma Bouaziz, Salwa Abid, Imen Graiet |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male DNA damage Cell Survival Health Toxicology and Mutagenesis p38 mitogen-activated protein kinases Apoptosis 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Pyrethrins medicine Humans Viability assay Pesticides 030304 developmental biology 0105 earth and related environmental sciences Aged chemistry.chemical_classification Aged 80 and over 0303 health sciences Reactive oxygen species Kinase Middle Aged Malondialdehyde HCT116 Cells Cell biology Mitochondria Comet assay chemistry Female Colorectal Neoplasms Oxidative stress DNA Damage |
| Zdroj: | Journal of toxicology and environmental health. Part A. 83(9) |
| ISSN: | 1528-7394 |
| Popis: | The widespread use of pesticides is beneficial for food production; however, there are numerous adverse consequences reported in the ecosystem and humans associated with exposure to these contaminants. The pyrethriod bifenthrin (BIF) is utilized for (1) maintenance, growth, and storage of agricultural products; (2) control of internal and external parasites of farm animals; and (3) eradication of insects threatening public health. Numerous data are available regarding environmental and ecological impact of pyrethriods on the central and peripheral nervous systems; however few studies focused on non-target tissues especially in humans. Therefore, the aim of this investigation was to determine the potential cytotoxic effects of BIF on a non-target tissue using human colorectal HCT-116 cells as a model. Data demonstrated that BIF reduced cell viability and disrupted mitochondrial functions which were accompanied by increased reactive oxygen species (ROS) levels indicating the presence of oxidative stress. BIF produced a significant elevation in levels of malondialdehyde (MDA) supporting the role of oxidative stress in pesticide-mediated toxicity. Concomitantly, a fall of mitochondrial transmembrane potential (Δψ), consequently producing perturbation of fluidity as well as excitability of cellular membranes was noted. Our results also indicated that BIF induced a rise in DNA damage as evidenced by the comet assay. An increase in mitogen-activated protein kinases (MAPKs), JNK (N-terminal Kinase), p38, and ERK (extracellular-signal-regulated kinase) suggested an apoptotic effect. Data thus indicated that BIF-induced cytotoxicity in human colorectal HCT-116 cells was associated with oxidative stress, mitochondrial dysfunction, and apoptosis. |
| Databáze: | OpenAIRE |
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