A Comparison of Libs for the Quantification of Au Nanoparticles using 1064 nm, 532 nm, and 266 nm Excitation
| Autor: | Aaleyah Joe, Cleon Barnett, Komal Vig |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Detection limit
0303 health sciences Materials science Analytical chemistry Atomic emission spectroscopy Biophysics Nanoparticle Laser law.invention Matrix (chemical analysis) 03 medical and health sciences 0302 clinical medicine law Colloidal gold Spectroscopy 030217 neurology & neurosurgery Excitation 030304 developmental biology |
| Zdroj: | Biophysical Journal. 110(3) |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2015.11.2637 |
| Popis: | Nanoparticles have emerged as a tool for wide range of medical and industrial applications. There are several methods that are used to characterize the size and distribution of the nanoparticles in cells and tissues. Laser-Induced Breakdown Spectroscopy (LIBS) is a versatile technique that has found its place in several analytical applications. Through short laser pulses, LIBS creates a plasma on the sample surface which yields critical elemental information. In this study we explore its use as method to estimate the concentration of gold nanoparticles in cells. We compare limit of detection (L.O.D.) obtained using plasmas produced by 15 mJ of laser energy at 1064 nm, 532 nm and 266 nm. Gold nanoparticles were incubated in human epithelial (HEp-2) cells at concentrations ranging from 0.30 to 1.25 µg/µl. Cells were trypsinzed and collected in HBSS after 24 hours incubation. 1.0 µl of cell incubated with nanoparticles were deposited on to pure silicon wafers and were analyzed using LIBS. The gold nanoparticle emission was monitored using the atomic emission line (Au I) at 267.59 nm. Under the current experimental conditions, the emission intensity emerging from the 266 nm laser produced plasmas was higher than the other two excitation wavelengths. In addition, using 266 nm as the excitation source led to improved L.O.D as compared to the other two wavelengths. Our results are promising towards using LIBS as a detection and quantification tools for nanoparticles in biological matrix. This work was supported by NSF-REU (DBI-1358923) to Dr. Komal Vig (PI) |
| Databáze: | OpenAIRE |
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