A derivative of Mycobacterium smegmatis mc2155 that lacks the duplicated chromosomal region
Autor: | Digby F. Warner, Gilles Etienne, Xiao-Ming Wang, Limenako G. Matsoso, Stephanie S. Dawes, Karine Soetaert, Neil G. Stoker, Jean Content, Valerie Mizrahi |
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Rok vydání: | 2006 |
Předmět: |
Microbiology (medical)
Genetics Mutation Surface Properties Mycobacterium smegmatis Immunology Mutant Biology biology.organism_classification medicine.disease_cause Polymerase Chain Reaction Microbiology Genome Open Reading Frames Transformation Genetic Infectious Diseases Plasmid Genes Bacterial Gene Duplication Gene duplication Chromosomal region medicine Gene Plasmids |
Zdroj: | Tuberculosis. 86:438-444 |
ISSN: | 1472-9792 |
Popis: | The genome of Mycobacterium smegmatis mc(2)155 contains a 56kb duplicated region. We isolated a mutant of mc(2)155 lacking this duplication (DeltaDRKIN). This mutation did not affect the growth rate, surface properties or transformation efficiency of the organism, confirming the potential utility of DeltaDRKIN for the study of genes contained within the duplicated region. |
Databáze: | OpenAIRE |
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