Extraction, purification, and structure characterization of polysaccharides from Crassostrea rivularis
| Autor: | Fan Xiuping, Lianyi Zhang, Qin Xiaoming, Jianjun Yuan, Zheng Huina, Zhang Chaohua |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Chromatography Rhamnose 010401 analytical chemistry Mannose 02 engineering and technology 021001 nanoscience & nanotechnology Polysaccharide 01 natural sciences 0104 chemical sciences Sepharose Gel permeation chromatography Crassostrea rivularis polysaccharide glycogen chemical characterization chemistry.chemical_compound Column chromatography chemistry Gas chromatography Cellulose 0210 nano-technology Food Science |
| Zdroj: | Food Science & Nutrition, 6(6):1621-1628 |
| Popis: | Crude polysaccharide was prepared from Crassostrea rivularis by 30% (w/v) potassium hydroxide solution at 90°C for 120 min. Three fractions (OG 1, OG 2, and OG 3) were purified by DEAE ‐52 cellulose and Sepharose 2B gel column chromatography. The chemical structures were determined using gas chromatography (GC ), high‐performance gel permeation chromatography (HPGPC ), Fourier‐transform infrared (FT ‐IR ) spectroscopy, and 1H and 13C nuclear magnetic resonance (NMR ) spectroscopy. The results indicated that OG 1 was composed of rhamnose and little mannose (8.71%), the ratio of Rha: Gal: Xyl: Fuc in OG 3 were 14:5.5:3:1. And their average molecular weights (Mw) were about 1.66 × 106 and 2.33 × 106 Da, respectively. OG 2 was composed only of glucose (98.23%), which means it was glycogen. OG 2 was consisted mainly of →4)‐ α‐D‐Glc‐(1→, with the branch chain every 6.5 glucose residues on average, which is →4,6)‐α‐D‐Glc‐(1→ and trace amount of α‐D‐Glc‐(1→ branched units. The Mw was 2.27 × 106 Da. It provides the bases for the bioactivity research. |
| Databáze: | OpenAIRE |
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