Response of liver to lipopolysaccharide treatment in male and female rats
| Autor: | Müjdat Uysal, Murat Giriş, Semra Doğru-Abbasoğlu, Bilge Depboylu, Vakur Olgaç |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Male Lipid Peroxides medicine.medical_specialty Necrosis Thiobarbituric acid medicine.medical_treatment Blotting Western Aspartate transaminase Apoptosis Toxicology medicine.disease_cause Pathology and Forensic Medicine chemistry.chemical_compound Bcl-2-associated X protein Internal medicine In Situ Nick-End Labeling medicine Animals Aspartate Aminotransferases Rats Wistar bcl-2-Associated X Protein chemistry.chemical_classification Sex Characteristics biology Caspase 3 Glutathione peroxidase Vitamin E Alanine Transaminase Cell Biology General Medicine Rats Endocrinology Liver Proto-Oncogene Proteins c-bcl-2 chemistry Alanine transaminase biology.protein Female medicine.symptom Oxidative stress |
| Zdroj: | Experimental and Toxicologic Pathology. 65:645-650 |
| ISSN: | 0940-2993 |
| DOI: | 10.1016/j.etp.2012.07.004 |
| Popis: | Gender is considered to be an important factor in endotoxin-induced tissue damage. Our aim was to examine the role of sex on the prooxidant-antioxidant status, necrotic and apoptotic events in the liver of lipopolysaccharide (LPS)-treated rats. We determined levels of lipid peroxides, non-enzymatic and enzymatic antioxidants, and expressions of apoptosis-related proteins, antiapoptotic B cell lymphoma-2 (Bcl-2) and proapoptotic Bax, caspase-3 activity and apoptotic cell numbers in the liver. Hepatic histopathology and serum alanine transaminase (ALT) and aspartate transaminase (AST) activities were also investigated. Male and female Wistar rats (180-200 g) were injected with LPS (10 mg/kg, i.p.) and examinations were performed 6 h after the injection. Significant increases in hepatic thiobarbituric acid reactive substances and diene conjugate levels were observed in male and female rats following LPS treatment. However, there were no changes in hepatic glutathione, vitamin E and vitamin C levels together with superoxide dismutase, glutathione peroxidase and glutathione transferase activities. LPS treatment caused significant increases in serum ALT and AST activities and lymphocyte infiltration and necrotic changes in the livers. Bcl-2 and Bax expressions, caspase-3 activity and apoptotic cell numbers were also found to be increased in both groups. In conclusion, no sex-dependent difference was observed in the changed hepatic prooxidant-antioxidant status of rats following LPS treatment. Besides, the process leading to apoptosis and necrosis in the liver showed a similar pattern in both gender of rats. |
| Databáze: | OpenAIRE |
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