High-Resolution Mass Spectrometry for the Measurement of PTH and PTH Fragments: Insights into PTH Physiology and Bioactivity

Autor:	Candice Z. Ulmer, Kittrawee Kritmetapak, Ravinder J. Singh, Hubert W. Vesper, Rajiv Kumar
Rok vydání:	2022
Předmět:	Chronic Kidney Disease-Mineral and Bone Disorder endocrine system Parathyroid Hormone Nephrology Humans General Medicine Renal Insufficiency Chronic Bone and Bones Mass Spectrometry Peptide Fragments hormones hormone substitutes and hormone antagonists
Zdroj:	Journal of the American Society of Nephrology. 33:1448-1458
ISSN:	1533-3450 1046-6673
DOI:	10.1681/asn.2022010036
Popis:	Full-length parathyroid hormone (PTH 1-84) is crucial for the regulation of calcium and phosphate homeostasis and bone remodeling. PTH 1-84 is metabolized into various PTH fragments, which are measured with varying levels of efficiency by PTH immunoassays. These PTH fragments, which increase in serum as CKD progresses, could potentially modulate the effects of PTH 1-84 and contribute to CKD-associated bone disorders. To obtain a true biologic representation of total PTH bioactivity, it is necessary to measure not only PTH 1-84 but also PTH fragments that are present in circulation. Traditional second-generation PTH immunoassays collectively measure PTH 1-84, PTH fragments, and post-translationally modified PTH 1-84, making it difficult to accurately predict the character of underlying renal osteodystrophy. This review highlights current advances in methods available for PTH measurement and the clinical relevance of PTH fragments in CKD. We emphasize the usefulness of mass spectrometry as a potential reference method for PTH measurement.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c412ecc835fb7c063ae822087e032a4 https://doi.org/10.1681/asn.2022010036 Zobrazit plný text záznamu