An Improved MLVF Method and Its Comparison with Traditional MLVF, spa Typing, MLST/SCCmec and PFGE for the Typing of Methicillin-Resistant Staphylococcus aureus

Autor: Hong-Yan Liang, Meng Xiao, Hui Zhang, Sun Hongli, Xiaofeng Jiang, Li Zhang, Guo-Yu Chen, Xue-Fei Du, Xiao-Yu Meng, Zhe Sun, Yue-Hong Jiang, Ya-Li Liu, Gui-Ling Zou, Ying-Chun Xu
Jazyk: angličtina
Rok vydání: 2014
Předmět:
DNA
Bacterial
Methicillin-Resistant Staphylococcus aureus
multilocus variable-number tandem repeat fingerprinting (MLVF)
pulse field gel electrophoresis (PFGE)
Multilocus sequence typing (MLST)
Biology
medicine.disease_cause
Article
Catalysis
Inorganic Chemistry
lcsh:Chemistry
Multiplex polymerase chain reaction
Pulsed-field gel electrophoresis
medicine
Typing
Physical and Theoretical Chemistry
Staphylococcal Protein A
Molecular Biology
Genotyping
lcsh:QH301-705.5
methicillin-resistant Staphylococcus aureus (MRSA)
genotyping
capillary gel electrophoresis
staphylococcal cassette chromosome mec (SCCmec)
Spectroscopy
Genetics
SCCmec
Organic Chemistry
Electrophoresis
Capillary
General Medicine
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
DNA Fingerprinting
Methicillin-resistant Staphylococcus aureus
Electrophoresis
Gel
Pulsed-Field
Computer Science Applications
DNA profiling
lcsh:Biology (General)
lcsh:QD1-999
Tandem Repeat Sequences
Multilocus sequence typing
Multiplex Polymerase Chain Reaction
Multilocus Sequence Typing
Zdroj: International Journal of Molecular Sciences, Vol 15, Iss 1, Pp 725-742 (2014)
International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 15; Issue 1; Pages: 725-742
ISSN: 1422-0067
Popis: Methicillin-resistant Staphylococcus aureus (MRSA) has become an important nosocomial pathogen, causing considerable morbidity and mortality. During the last 20 years, a variety of genotyping methods have been introduced for screening the prevalence of MRSA. In this study, we developed and evaluated an improved approach capillary gel electrophoresis based multilocus variable-number tandem-repeat fingerprinting (CGE/MLVF) for rapid MRSA typing. A total of 42 well-characterized strains and 116 non-repetitive clinical MRSA isolates collected from six hospitals in northeast China between 2009 and 2010 were tested. The results obtained by CGE/MLVF against clinical isolates were compared with traditional MLVF, spa typing, Multilocus sequence typing/ staphylococcal cassette chromosome mec (MLST/SCCmec) and pulse field gel electrophoresis (PFGE). The discriminatory power estimated by Simpson’s index of diversity was 0.855 (28 types), 0.855 (28 patterns), 0.623 (11 types), 0.517 (8 types) and 0.854 (28 patterns) for CGE/MLVF, traditional MLVF, spa typing, MLST/SCCmec and PFGE, respectively. All methods tested showed a satisfied concordance in clonal complex level calculated by adjusted Rand’s coefficient. CGE/MLVF showed better reproducibility and accuracy than traditional MLVF and PFGE methods. In addition, the CGE/MLVF has potential to produce portable results. In conclusion, CGE/MLVF is a rapid and easy to use MRSA typing method with lower cost, good reproducibility and high discriminatory power for monitoring the outbreak and clonal spread of MRSA isolates.
Databáze: OpenAIRE
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