Oxidative Stress as a Contributor to Insulin Resistance in the Skeletal Muscles of Mice with Polycystic Ovary Syndrome
| Autor: | Qiyang Yao, Xin Zou, Shihe Liu, Haowen Wu, Qiyang Shen, Jihong Kang |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Catalysis
Antioxidants Inorganic Chemistry Mice polycystic ovary syndrome oxidative stress insulin resistance skeletal muscle Animals Humans Testosterone Physical and Theoretical Chemistry Muscle Skeletal Molecular Biology Spectroscopy Organic Chemistry General Medicine Dehydroepiandrosterone Computer Science Applications Acetylcysteine Mice Inbred C57BL Oxidative Stress Receptors Androgen Female Insulin Resistance Oxidoreductases Reactive Oxygen Species Biomarkers NADP Polycystic Ovary Syndrome |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11384 |
| ISSN: | 1422-0067 |
| Popis: | Polycystic ovarian syndrome (PCOS) is a reproductive, endocrine, and metabolic disorder. Circulating markers of oxidative stress are abnormal in women with PCOS. There is a close relationship between oxidative stress and insulin resistance (IR). However, little information is available about oxidative stress in the skeletal muscles of those affected by PCOS. In this study, PCOS was induced in prepubertal C57BL/6J mice by injection with dehydroepiandrosterone. Oxidative stress biomarkers were then measured in both serum and skeletal muscles. The underlying mechanisms were investigated in C2C12 myotubes treated with testosterone (T). We discovered increased oxidative biomarkers, increased ROS production, and damaged insulin sensitivity in the skeletal muscles of mice with PCOS. High levels of T caused mitochondrial dysfunction and increased ROS levels through the androgen receptor (AR)-nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) signaling pathway in C2C12 cells. Treatment of C2C12 cells with an antioxidant N-acetylcysteine (NAC) decreased T-induced ROS production, improved mitochondrial function, and reversed IR. Administration of NAC to mice with PCOS improved insulin sensitivity in the skeletal muscles of the animals. Hyperandrogenism caused mitochondrial dysfunction and redox imbalance in the skeletal muscles of mice with PCOS. We discovered that oxidative stress contributed to skeletal muscle IR in PCOS. Reducing ROS levels may improve the insulin sensitivity of skeletal muscles in patients with PCOS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|