Pigmentiphaga humi sp. nov., isolated from soil amended with humic acid
| Autor: | Alexis Criscuolo, Stefanie P. Glaeser, Hans-Jürgen Busse, Chantal Bizet, Peter Kämpfer, Dominique Clermont, John A. McInroy |
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| Přispěvatelé: | Justus-Liebig-Universität Gießen = Justus Liebig University (JLU), Veterinärmedizinische Universität Wien, Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Collection de l'Institut Pasteur (CIP), Institut Pasteur [Paris] (IP), Centre de Ressources biologiques de l'Institut Pasteur - Biological Resource Center of the Institut Pasteur (CRBIP), Auburn University (AU), This work received no specific grant from any funding agency, We thank Katja Grebing, Maria Sowinsky, and Gundula Will of the Institute of Applied Microbiology, JLU Giessen, for excellent technical assistance. We also thank the ‘P2M-Plateforme de Microbiologie Mutualisée’ (PIBnet) from Institut Pasteur for genomic sequencing, Justus-Liebig-Universität Gießen (JLU), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine MESH: Sequence Analysis DNA Ubiquinone [SDV]Life Sciences [q-bio] Diaminopimelic Acid 01 natural sciences chemistry.chemical_compound Genus RNA Ribosomal 16S MESH: Ubiquinone MESH: Putrescine MESH: Phylogeny Phospholipids Phylogeny Soil Microbiology Base Composition biology Strain (chemistry) Fatty Acids General Medicine Bacterial Typing Techniques Pigmentiphaga MESH: Fatty Acids MESH: RNA Ribosomal 16S Pigmentiphaga kullae [SDE]Environmental Sciences Alabama DNA Bacterial MESH: Humic Substances Diamino acid 010603 evolutionary biology Microbiology MESH: Bacterial Typing Techniques 03 medical and health sciences MESH: Base Composition Putrescine MESH: Diaminopimelic Acid Pigmentiphaga humi Humic Substances Ecology Evolution Behavior and Systematics MESH: Phospholipids Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification MESH: DNA Bacterial MESH: Alabama 030104 developmental biology MESH: Alcaligenaceae MESH: Soil Microbiology chemistry Peptidoglycan Bacteria Alcaligenaceae |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (6), pp.1573-1578. ⟨10.1099/ijsem.0.003355⟩ International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2019, 69 (6), pp.1573-1578. ⟨10.1099/ijsem.0.003355⟩ |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003355 |
| Popis: | International audience; A slightly beige-pigmented, Gram-stain-negative, rod-shaped bacterium, strain IMT-318T, was isolated from soil in a field located in Malvern, Alabama, USA. Phylogenetic analysis based on the 16S rRNA gene placed the strain within the genus Pigmentiphaga with highest 16S rRNA gene sequence similarity of 98.74 % and 98.67 % to the type strains of Pigmentiphaga kullae and Pigmentiphaga daeguensis, respectively. Sequence similarities to all other species of the genus were below 98.0 %. Results of the chemotaxonomic analysis, however, showed clear similarities to the genus Pigmentiphaga. The main cellular fatty acids of the strain were C16 : 0, C18 : 1 ω7c, C17 : 0 cyclo and C19 : 0 cyclo ω8c. The major quinone was ubiquinone Q-8. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified aminophospholipid. In the polyamine pattern, putrescine and 2-hydroxyputrescine were predominant. The diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phylogenetic, chemotaxonomic and phenotypic analyses, we propose a new species of the genus Pigmentiphaga, with the name Pigmentiphaga humi sp. nov. and strain IMT-318T (=LMG 30658T=CIP 111626T=CCM 8859T) as the type strain. |
| Databáze: | OpenAIRE |
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