Sequence, structure and evolution of the ecdysone-inducible Lsp-2 gene of Drosophila melanogaster
| Autor: | Thorsten Burmester, Sylvie Mousseron-Grall, Jean-Antoine Lepesant, Etienne Delain, Martine Barray, Carol Chihara, Raymond Pictet, Jana Kejzlarova-Lepesant |
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| Rok vydání: | 1997 |
| Předmět: |
Signal peptide
Ecdysone Protein subunit Molecular Sequence Data Genes Insect Biochemistry Molecular evolution parasitic diseases Animals Drosophila Proteins Amino Acid Sequence Codon Gene Protein secondary structure Phylogeny Genetics biology Molecular mass Base Sequence fungi Intron biology.organism_classification TATA Box Microscopy Electron Drosophila melanogaster Enzyme Induction Insect Proteins |
| Zdroj: | European journal of biochemistry. 245(1) |
| ISSN: | 0014-2956 |
| Popis: | The Lsp-2 gene encodes a major larval serum protein (hexamerin) of Drosophila melanogaster. Transcription of Lsp-2 is controlled by 20-hydroxyecdysone. Here we report the analysis of the structure of the Lsp-2 gene including the adjacent 5′ and 3′ sequences. In contrast to all other known hexamerin genes, Lsp-2 does not contain an intron. The Lsp-2 mRNA measures 2312 bases, as deduced from experimental determination of the transcription-start and stop sites and conceptual translation results in a 718 amino acid hexamerin subunit, including a 21-amino-acid signal peptide. While the calculated molecular mass of the native 697-amino-acid subunit is 83.5 kDa, mass spectrometry gave a value of 74.5 kDa. We detected in the Lsp-2 gene a 2052-bp antisense ORF that probably does not code for any protein. An unusual accumulation of rarely used codon triplets was found at the 5′ and 3′ ends of the Lsp-2 ORF. The calculated secondary structure matches well with that of arthropod hemocyanins. Electron micrographs show for LSP-2 hexamers a cubic shape, which can not be easily reconciled with its hexameric structure. Phylogenetic analysis revealed that LSP-2 diverged from the LSP-1-like hexamerins after separation of the Diptera from other insect orders. |
| Databáze: | OpenAIRE |
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