Discovery and Identification of Potential Biomarkers in a Prospective Study of Chronic Lymphoid Malignancies Using SELDI-TOF−MS
| Autor: | Noelle Potier, Laurent Miguet, Laurent Mauvieux, Raoul Herbrecht, Ralf Bogumil, Alain Van Dorsselaer, Philippe Decloquement |
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| Přispěvatelé: | Chimie de la matière complexe (CMC), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Lymphoma B-Cell Molecular Sequence Data Protein Array Analysis Diagnostic tools Biochemistry Mass Spectrometry 03 medical and health sciences 0302 clinical medicine Immunophenotyping [CHIM.ANAL]Chemical Sciences/Analytical chemistry SELDI-TOF-MS medicine Amino Acid Sequence Prospective cohort study Chromatography High Pressure Liquid ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Blood Proteins General Chemistry 3. Good health Protein profiling Transthyretin Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization 030220 oncology & carcinogenesis Potential biomarkers Immunology biology.protein Biomarker (medicine) Electrophoresis Polyacrylamide Gel Biomarkers |
| Zdroj: | Journal of Proteome Research Journal of Proteome Research, American Chemical Society, 2006, 5 (9), pp.2258-2269. ⟨10.1021/pr060058y⟩ |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | The accurate diagnosis of the different forms of chronic mature B-cell lymphocytic malignancies is of primary importance to determine an appropriate and efficient treatment. Usually, the diagnosis is achieved by morphology and immunophenotyping. Nevertheless, the diagnostic tools available are not able to discriminate pathologies with variable evolution, or to classify some of them. To discover new biomarkers, we used peptide and protein profiling SELDI-TOF-MS, to analyze 39 chronic B-cell malignancies and 20 control serum samples. Markers of interest were subsequently identified and characterized. In the obtained SELDI-MS profiles, most of the differences were observed in three mass ranges (m/z = 13 000; m/z = 9000; m/z2000). Identification of these biomarkers was achieved either by direct enrichment on the ProteinChip arrays followed by on-chip-MS/MS or by chromatographic fractionation, 1D-gel followed by nanoLC-MS/MS analysis. An increase of a sulfite form of transthyretin (13,841 Da) was observed in the patient group. A second set of markers at 8.6 and 8.9 kDa was identified as complement related fragment proteins, the C3a and C4a anaphylatoxins. In the low mass range, several peptides originating from N-terminal and C-terminal processing of the C3 alpha and C4 alpha chains were specifically observed in 38% of the patient sera, but in none of the control sera. This study emphasizes the usefulness of mass spectrometry studies in such malignancies. |
| Databáze: | OpenAIRE |
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