COMPARTMENTS AND CELL FLOWS WITHIN THE MOUSE HAEMOPOIETIC SYSTEM II. ESTIMATED RATES OF INTERCHANGE
| Autor: | C. E. Ford, D. A. Ogden, H. S. Micklem, E. P. Evans, J. G. Gray |
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| Rok vydání: | 1975 |
| Předmět: |
Male
Hematopoietic System medicine.medical_treatment Splenectomy Population Bone Marrow Cells Thymus Gland Biology Andrology Mice Bone Marrow Cell Movement medicine Animals Progenitor cell education Mitosis Bone Marrow Transplantation education.field_of_study Cell Biology General Medicine Hematopoietic Stem Cells Radiation Injuries Experimental Haematopoiesis medicine.anatomical_structure Immunology Mice Inbred CBA Female Lymph Nodes Bone marrow Lymph Stem cell |
| Zdroj: | Cell Proliferation. 8:233-248 |
| ISSN: | 1365-2184 0960-7722 |
| Popis: | Part-body irradiated CBA mice were injected with CBA-T6 bone marrow. In this way a predominantly donor population was established in the femora while the marrow of the humeri remained largely (average 94%) of host origin. In animals examined cytologically up to 2 years later, no tendency was observed for the proportion of donor cells in the humeri to increase. Splenectomy had no effect on this. When femoral bone marrow from the experimental mice was injected into lethally (whole-body) irradiated recipients, cells originating from the primary host repopulated the lymph nodes to a disproportionate extent. Equilibration between the cell populations of femora and humeri occurred after re-exposure to 600 rad whole-body irradiation, but not after 100 rad or 350 rad; thus, regeneration of damaged bone marrow involved a significant contribution from extrinsic stem cells only after the highest dose of radiation. The data are compatible with an inflow of at most ten effective stem cells per humerus per day from the blood, and suggest a much lower figure. This means that few if any of the stem cells of peripheral blood enter the bone marrow and found haemopoietic clones. Evidence is adduced for the existence of a proliferating lymphoid sub-population in the bone marrow, contributing some 5-10% of the observed mitoses. The mitotic cells in the lymph nodes are replaced from marrow-derived progenitors at an estimated rate of 4-5%/day. The relevant data for the thymus are more variable, but suggest an average figure of 8-11%/day. Earlier data from mouse parabionts suggest a lower rate of inflow to the thymus. |
| Databáze: | OpenAIRE |
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