The ACBP gene family in Rhodnius prolixus : Expression, characterization and function of RpACBP-1
| Autor: | Katia C. Gondim, David Majerowicz, Hans Kristian Hannibal-Bach, Luciano Aparecido Meireles Grillo, Claudio A. Masuda, Nils J. Færgeman, Michele Alves-Bezerra, Bruno L. Bozaquel-Morais, Jens Knudsen, Rodolfo S C Castro |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Oviposition Fat Body Mutant Biochemistry 03 medical and health sciences Gene expression Journal Article Animals Gene family Rhodnius prolixus Molecular Biology Gene Diazepam Binding Inhibitor Gene knockdown 030102 biochemistry & molecular biology biology Research Support Non-U.S. Gov't Insect physiology Lipid metabolism Lipid Metabolism biology.organism_classification Fertility 030104 developmental biology Gene Expression Regulation Rhodnius Insect Science Insect Proteins Female RNA Interference lipids (amino acids peptides and proteins) Acyl Coenzyme A |
| Zdroj: | Majerowicz, D, Hannibal-Bach, H K, Castro, R S C, Bozaquel-Morais, B L, Alves-Bezerra, M, Grillo, L A M, Masuda, C A, Færgeman, N J, Knudsen, J & Gondim, K C 2016, ' The ACBP gene family in Rhodnius prolixus : Expression, characterization and function of RpACBP-1 ', Insect Biochemistry and Molecular Biology, vol. 72, pp. 41-52 . https://doi.org/10.1016/j.ibmb.2016.03.002 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/j.ibmb.2016.03.002 |
| Popis: | The acyl-CoA-binding proteins (ACBP) constitute a family of conserved proteins that bind acyl-CoA with high affinity and protect it from hydrolysis. Thus, ACBPs may have essential roles in basal cellular lipid metabolism. The genome of the insect Rhodnius prolixus encodes five ACBP genes similar to those described for other insect species. The qPCR analysis revealed that these genes have characteristic expression profiles in insect organs, suggesting that they have specific roles in insect physiology. Recombinant RpACBP-1 was able to bind acyl-CoA in an in vitro gel-shift assay. Moreover, heterologous RpACBP-1 expression in acb1Δ mutant yeast rescued the multi-lobed vacuole phenotype, indicating that RpACBP-1 acts as a bona fide acyl-CoA-binding protein. RpACBP-1 knockdown using RNAi caused triacylglycerol accumulation in the insect posterior midgut and a reduction in the number of deposited eggs. The amount of stored triacylglycerol was reduced in flight muscle, and the incorporation of fatty acids in cholesteryl esters was increased in the fat body. These results showed that RpACBP-1 participates in several lipid metabolism steps in R. prolixus. |
| Databáze: | OpenAIRE |
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