Expressed Sequence Tag Analysis of the Human Pathogen Paracoccidioides brasiliensis Yeast Phase: Identification of Putative Homologues of Candida albicans Virulence and Pathogenicity Genes
| Autor: | Maria Helena S. Goldman, Everaldo dos Reis Marques, Ana Paula Bogossian, Regina Costa de Oliveira, Fredj Tekaia, Marina P. Nobrega, Luiz R. Nunes, Patrícia Marostica Vitorelli, Camile P. Semighini, Marcela Savoldi, Luciano Ângelo de Souza Bernardes, Andréa Carla Quiapin, Leslie Ecker Ferreira, Gustavo H. Goldman, Rosana Puccia, Diógenes Custódio Duarte Ribeiro, Júlio C. Moreira, Luiz R. Travassos, Wagner L. Batista, Francisco G. Nobrega |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Complementary Virulence Microbiology Gene Expression Regulation Enzymologic Gene Expression Regulation Fungal Candida albicans medicine Humans RNA Messenger Molecular Biology Gene Expressed Sequence Tags Paracoccidioides brasiliensis Expressed sequence tag Base Sequence Mycelium biology Paracoccidioidomycosis Candidiasis Paracoccidioides Articles General Medicine Isocitrate lyase biology.organism_classification medicine.disease Yeast Enzymes Genome Fungal |
| Zdroj: | Eukaryotic Cell. 2:34-48 |
| ISSN: | 1535-9786 1535-9778 |
| Popis: | Paracoccidioides brasiliensis , a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here a survey of expressed genes in the yeast pathogenic phase of P. brasiliensis . We obtained 13,490 expressed sequence tags from both 5′ and 3′ ends. Clustering analysis yielded the partial sequences of 4,692 expressed genes that were functionally classified by similarity to known genes. We have identified several Candida albicans virulence and pathogenicity homologues in P. brasiliensis . Furthermore, we have analyzed the expression of some of these genes during the dimorphic yeast-mycelium-yeast transition by real-time quantitative reverse transcription-PCR. Clustering analysis of the mycelium-yeast transition revealed three groups: (i) RBT, hydrophobin, and isocitrate lyase; (ii) malate dehydrogenase, contigs Pb1067 and Pb1145, GPI, and alternative oxidase; and (iii) ubiquitin, delta-9-desaturase, HSP70, HSP82, and HSP104. The first two groups displayed high mRNA expression in the mycelial phase, whereas the third group showed higher mRNA expression in the yeast phase. Our results suggest the possible conservation of pathogenicity and virulence mechanisms among fungi, expand considerably gene identification in P. brasiliensis , and provide a broader basis for further progress in understanding its biological peculiarities. |
| Databáze: | OpenAIRE |
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