Histone H4 acetylation required for chromatin decompaction during DNA replication
| Autor: | Ruan, K., Yamamoto, T. G., Asakawa, H., Chikashige, Y., Kimura, Hiroshi, Masukata, H., Haraguchi, T., Hiraoka, Y. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
DNA Replication
Biology Chromatin remodeling Article S Phase Histone H4 Histones Control of chromosome duplication Histone H1 Histone H2A Schizosaccharomyces Histone code Nucleosome DNA Fungal Histones/genetics/*metabolism Schizosaccharomyces pombe Proteins/genetics/*metabolism Multidisciplinary Acetylation Schizosaccharomyces/genetics/*metabolism Chromatin Assembly and Disassembly S Phase/physiology Chromatin Chromatin Assembly and Disassembly/*physiology DNA Replication/*physiology Biochemistry DNA Fungal/*biosynthesis/genetics Schizosaccharomyces pombe Proteins |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep12720 |
| Popis: | Faithful DNA replication is a prerequisite for cell proliferation. Several cytological studies have shown that chromosome structures alter in the S-phase of the cell cycle. However, the molecular mechanisms behind the alteration of chromosome structures associated with DNA replication have not been elucidated. Here, we investigated chromatin structures and acetylation of specific histone residues during DNA replication using the meiotic nucleus of the fission yeast Schizosaccharomyces pombe. The S. pombe meiotic nucleus provides a unique opportunity for measuring the levels of compaction of chromatin along the chromosome in a defined orientation. By direct measurement of chromatin compaction in living cells, we demonstrated that decompaction of chromatin occurs during meiotic DNA replication. This chromatin decompaction was suppressed by depletion of histone acetyltransferase Mst1 or by arginine substitution of specific lysine residues (K8 and K12) of histone H4. These results suggest that acetylation of histone H4 residues K8 and K12 plays a critical role in loosening chromatin structures during DNA replication. |
| Databáze: | OpenAIRE |
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