Glycated human serum albumin induces NF-κB activation and endothelial nitric oxide synthase uncoupling in human umbilical vein endothelial cells
| Autor: | Bruno K. Rodiño-Janeiro, José Ramón González-Juanatey, Mercedes González-Peteiro, Ezequiel Álvarez, Sergio Raposeiras-Roubín, Beatriz Paradela-Dobarro |
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| Rok vydání: | 2015 |
| Předmět: |
Glycation End Products
Advanced Endothelium Nitric Oxide Synthase Type III Cell Survival Endocrinology Diabetes and Metabolism Serum albumin Serum Albumin Human Nitric Oxide Gene Expression Regulation Enzymologic Nitric oxide chemistry.chemical_compound Endocrinology Enos Superoxides Internal Medicine medicine Human Umbilical Vein Endothelial Cells Humans Glycated Serum Albumin Enzyme Inhibitors Cells Cultured Serum Albumin Protein Synthesis Inhibitors NADPH oxidase biology NOX4 NADPH Oxidases NF-kappa B p50 Subunit biology.organism_classification Cell biology Transcription Factor AP-1 Oxidative Stress medicine.anatomical_structure Biochemistry chemistry NADPH Oxidase 4 biology.protein P22phox Endothelium Vascular Reactive Oxygen Species Diabetic Angiopathies |
| Zdroj: | RUNA. Repositorio da Consellería de Sanidade e Sergas Servizo Galego de Saúde (SERGAS) |
| Popis: | Aims Non-enzymatic glycated proteins could mediate diabetes vascular complications, but the molecular mechanisms are unknown. Our objective was to find new targets involved in the glycated human serum albumin (gHSA)-enhanced extracellular reactive oxygen species (ROS) production in human endothelial cells. Methods & results Some nuclear factors and phosphorylation cascades were analysed. gHSA activated nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), which up-regulated NOX4 and P22PHOX and enhanced ROS production. Pharmacological inhibition of NF-κB reversed gHSA-enhanced NOX4 expression and decreased gHSA-induced ROS production in extra- and intracellular spaces. The inhibition of activator protein-1 (AP-1) induced a rise in NOX4 and P22PHOX subunit expression and a down-regulation of endothelial nitric oxide synthase (eNOS). AP-1 inhibition also enhanced extracellular ROS production in the presence of serum albumin, but not with gHSA. These results were explained by the eNOS uncoupling induced by gHSA, also demonstrated in this study. Phosphatidylinositol 3-kinase or mitogen-activated protein kinase kinase 1/2 did not show to be involved in gHSA-induced ROS production. Conclusions All together, the results suggested that gHSA-enhanced ROS production in endothelium is mediated by: 1) NF-κB activation and subsequence up-regulation of NADPH oxidase, 2) eNOS uncoupling. AP-1, although is not directly affected by gHSA, is another target for regulating NADPH oxidase and eNOS expression in endothelial cells. |
| Databáze: | OpenAIRE |
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