Differential effects of interleukin-4 in peptide induced autoimmunity
| Autor: | Morris Edelman, Bisram Deocharan, Chaim Putterman, Paola Marambio |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Kidney Glomerulus
Immunology Autoimmunity Biology medicine.disease_cause Histones Mice Antigen medicine Animals Lupus Erythematosus Systemic Immunology and Allergy Cells Cultured Interleukin 4 Autoantibodies Mice Knockout Autoimmune disease Mice Inbred BALB C Systemic lupus erythematosus Autoantibody Wild type DNA medicine.disease Immunohistochemistry Disease Models Animal Immunoglobulin M Immunoglobulin G biology.protein Female Interleukin-4 Laminin Antibody Peptides Spleen |
| Zdroj: | Clinical Immunology. 108:80-88 |
| ISSN: | 1521-6616 |
| Popis: | BALB/c mice immunized with multimeric DWEYSVWLSN develop IgG1 anti-DNA antibodies and glomerular immunoglobulin deposits, leading us to investigate the role of IL-4 in this model of antigen induced lupus. Splenocytes from DWEYSVWLSN immunized mice secreted IL-4 but not γ-interferon. Following peptide immunization, IgG1 anti-peptide and anti-DNA antibodies were significantly higher in IL-4 wild type mice, while IgM and IgG3 anti-DNA levels were significantly higher in IL-4 knockout mice. Titers of IgG anti-laminin and anti-histone, but not anti-Sm/RNP and anti-cardiolipin antibodies, were significantly higher in the IL-4 wild type group. Glomerular immunoglobulin deposition was substantially decreased in IL-4 knockout mice. We conclude that while IL-4 does not materially affect the generation of some autoantibody responses associated with peptide induced autoimmunity, IL-4 deficiency inhibits kidney immunoglobulin deposition. The effect of IL-4 on humoral autoimmunity in lupus is complex, and is dependent on genetic background, the antigenic trigger and stage of disease. |
| Databáze: | OpenAIRE |
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