Gentamicin-Loaded Polysaccharide Membranes for Prevention and Treatment of Post-operative Wound Infections in the Skeletal System
| Autor: | Monika Brzychczy-Włoch, Krzysztof Pietryga, Wojciech Chrzanowski, Elżbieta Pamuła, Małgorzata Krok-Borkowicz, Łucja Rumian, Dominika Kulig, Urszula Cibor |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus Alginates Pharmaceutical Science chemistry.chemical_element 02 engineering and technology Calcium Polysaccharide 03 medical and health sciences chemistry.chemical_compound Pharmacokinetics Glucuronic Acid Polysaccharides Materials Testing medicine Humans Pharmacology (medical) Post operative Pharmacology chemistry.chemical_classification Drug Carriers Hexuronic Acids Organic Chemistry Polysaccharides Bacterial Membranes Artificial Osteomyelitis 021001 nanoscience & nanotechnology Antimicrobial Gellan gum Anti-Bacterial Agents Drug Liberation 030104 developmental biology Membrane Cross-Linking Reagents chemistry Biochemistry Wound Infection Molecular Medicine Gentamicin Collagen Gentamicins 0210 nano-technology Biotechnology Nuclear chemistry medicine.drug |
| Popis: | To develop polysaccharide-based membranes that allow controlled and localized delivery of gentamicin for the treatment of post-operative bone infections. Membranes made of gellan gum (GUM), sodium alginate (ALG), GUM and ALG crosslinked with calcium ions (GUM + Ca and ALG + Ca, respectively) as well as reference collagen (COL) were produced by freeze-drying. Mechanical properties, drug release, antimicrobial activity and cytocompatibility of the membranes were assessed. The most appropriate handling and mechanical properties (Young’s modulus, E = 92 ± 4 MPa and breaking force, F MAX = 2.6 ± 0.1 N) had GUM + Ca membrane. In contrast, COL membrane showed F MAX = 0.14 ± 0.02 N, E = 1.0 ± 0.3 MPa and was deemed to be unsuitable for antibiotic delivery. The pharmacokinetic data demonstrated a uniform and sustainable delivery of gentamicin from GUM + Ca (44.4 ± 1.3% within 3 weeks), while for COL, ALG and ALG + Ca membranes the most of the drug was released within 24 h (55.3 ± 1.9%, 52.5 ± 1.5% and 37.5 ± 1.8%, respectively). Antimicrobial activity against S. aureus and S. epidermidis was confirmed for all the membranes. GUM + Ca and COL membranes supported osteoblasts growth, whereas on ALG and ALG + Ca membranes cell growth was reduced. GUM + Ca membrane holds promise for effective treatment of bone infections thanks to favorable pharmacokinetics, bactericidal activity, cytocompatibility and good mechanical properties. |
| Databáze: | OpenAIRE |
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