Antiproliferative Activity and Apoptosis Induction of Eucalyptus Citriodora Resin and Its Major Bioactive Compound in Melanoma B16F10 Cells
| Autor: | Heuy-Ling Chu, Ling-Feng Kuo, Ya-Ting Wang, Shwu-Woan Lee, Pin-Der Duh, Tsuey-Pin Lin, Wen-Jye Yen, Zong-Tsi Chen |
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| Rok vydání: | 2012 |
| Předmět: |
Melanoma
Experimental Ethyl acetate Apoptosis Aromadendrin medicine.disease_cause Nitric oxide Mice chemistry.chemical_compound Cell Line Tumor medicine Animals MTT assay Cell Proliferation bcl-2-Associated X Protein Membrane Potential Mitochondrial Eucalyptus Caspase 3 Chemistry General Chemistry Bioactive compound Biochemistry Eucalyptus citriodora Methacrylates General Agricultural and Biological Sciences Resins Plant Oxidative stress |
| Zdroj: | Journal of Agricultural and Food Chemistry. 60:7866-7872 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf301068z |
| Popis: | Antiproliferative activity and apoptosis induction of ethyl acetate of Eucalyptus citriodora resin (EAEER), and its major bioactive compound in melanoma B16F10 cells were investigated. 6-[1-(p-Hydroxy-phenyl)ethyl]-7-O-methyl aromadendrin (HEMA), a flavanol derivative, was isolated from EAEER and identified on the basis of its mass and NMR spectra. The results from MTT assay showed high antiproliferative effects of EAEER and HEMA on B16F10 cells. Moreover, EAEER- and HEMA-induced cell apoptosis was association with the decrease in the mitochondrial transmembrane potentials (Δψ(m)), increase in Bax/Bcl-2 ratio, and activation of caspase-3. Cells treated with EAEER and HEMA generated intracellular reactive oxygen species (ROS) and nitric oxide (NO), indicating that ROS and RNS play important roles in the induction of apoptosis in B16F10 cells. Taken together, EAEER and its major bioactive compound, HEMA, inhibited the proliferation of B16F10 cells via apoptosis and may be a potential antimelanoma agent. |
| Databáze: | OpenAIRE |
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