Activation of c-K-ras mutations in human gastrointestinal tumors
| Autor: | Itzhak Shapira, Zamir Halpern, Menhachem Moshkowitz, Marilie D. Gammon, Baruch S. Stern, Jaccob Ratan, Nadir Arber, Ina Fabian, Hanina Hibshoosh |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adenoma
medicine.medical_specialty Esophageal Neoplasms Cell DNA Mutational Analysis Biology Adenocarcinoma medicine.disease_cause Gastroenterology Polymerase Chain Reaction law.invention Pathogenesis Barrett Esophagus law Stomach Neoplasms Internal medicine Intestinal Neoplasms medicine Humans Point Mutation Esophagus Stage (cooking) Israel Polymerase chain reaction Alleles Gastrointestinal Neoplasms Mutation Polymorphism Genetic Hepatology Histology digestive system diseases medicine.anatomical_structure Genes ras Carcinoma Squamous Cell New York City Restriction fragment length polymorphism |
| Zdroj: | Gastroenterology. 118(6) |
| ISSN: | 0016-5085 |
| Popis: | Background & Aims: Ras genes are the most frequently detected oncogenes in human malignancies. Data regarding the frequency of c-K- ras mutations in esophageal, gastric, and small bowel tumors are limited and controversial. Methods: DNA was extracted from 262 formalin-fixed, paraffin-embedded sections of gastrointestinal samples and tumors, including Barrett's esophagus, esophageal squamous cell carcinomas and adenocarcinomas, and small and large bowel adenomas and adenocarcinomas. The presence of c-K- ras codon 12 mutations was determined using a nonradioactive polymerase chain reaction–based restriction fragment length polymorphism assay. Results: c-K- ras mutations were detected in 1 of 39 (2%) patients with Barrett's esophagus, 1 of 21 (5%) adenocarcinomas, 0 of 27 squamous cell carcinomas of the esophagus, and 1 of 32 (3%) gastric adenocarcinomas. It was also present in 8 of 20 (40%) and 10 of 28 (36%) small bowel adenomas and adenocarcinomas, respectively. Similar numbers were observed in 10 of 25 (40%) large bowel adenomas and 11 of 30 adenocarcinomas (37%). Mutations were not associated with age, gender, histology, grade, stage, location, or mortality. Conclusions: The frequency of codon 12 c-K- ras mutations in small and large bowel tumors is approximately 10-fold higher than that of tumors in the upper gastrointestinal tract. GASTROENTEROLOGY 2000;118:1045-1050 |
| Databáze: | OpenAIRE |
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