Restriction Fragment Length Polymorphism Analyses of Iranian Strains of Xanthomonas campestris from Cereals and Grasses
| Autor: | A. Alizadeh, M Arlat, C A Boucher, G. Barrault, A. Sarrafi |
|---|---|
| Přispěvatelé: | Laboratoire de Biologie moléculaire des relations plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration |
| Rok vydání: | 2019 |
| Předmět: |
Genetics
biology Hybridization probe food and beverages Plant Science biology.organism_classification Xanthomonas campestris [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy genomic DNA Genetic marker Typing Genetic variability Restriction fragment length polymorphism Agronomy and Crop Science [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy Bacterial leaf streak |
| Zdroj: | Plant Disease Plant Disease, American Phytopathological Society, 1995, 79 (1), 7 p |
| ISSN: | 0191-2917 |
| Popis: | Restriction fragment length polymorphism (RFLP) analyses of the genomic DNA of 45 Xanthomonas campestris strains from cereals and grasses in Iran, and of 17 reference strains, were performed using two probes originating from X. campestris and including hrp genes. The Iranian strains studied belonged to three clearly distinct RFLP groups related to the grouping previously established on the basis of biochemical and physiological characters and host range. RFLP group 1 encompassed all the strains pathogenic to barley but not to the other plants tested (i.e., wheat, rye, Bromus inermis, Lolium multiflorum, Agropyron elongatum, and oat). RFLP group 2 contained strains that are pathogenic to all the above mentioned plants except oat. One strain, which has the same host range as group 2, was classified as RFLP group 3. Reference strains were distributed over these three groups, independently of their geographical origin. Strains in groups 1 and 3 had highly conserved RFLP patterns. In contrast, group 2 strains were easily split into two RFLP subgroups, although they did not differ significantly for other characters. The data suggest that RFLP analysis is a useful tool to distinguish among X. campestris strains causing bacterial leaf streak of cereals. |
| Databáze: | OpenAIRE |
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