Validation of an in vitro method to determine infectivity of cryopreserved sporozoites in stabilates of Theileria spp
| Autor: | Gwen Wilkie, E. Kirvar, C.G.D. Brown |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Spores Serial dilution Theileria parva Tick Sensitivity and Specificity Peripheral blood mononuclear cell Cryopreservation Ticks Theileria parasitic diseases Animals Cells Cultured Infectivity Sheep General Veterinary biology Reproducibility of Results General Medicine biology.organism_classification Virology In vitro Theileriasis Leukocytes Mononuclear Cattle Female Parasitology |
| Zdroj: | Veterinary Parasitology. 104:199-209 |
| ISSN: | 0304-4017 |
| Popis: | The infectivity of 15 cryopreserved Theileria spp. sporozoite stabilates was assessed semi-quantitatively by titration using naive peripheral blood mononuclear cells (PBM) in vitro in multi-well plates. Using the method described, the effective dilution, which would result in 50% of replicate wells infected (ED50), was calculated. The ED50 for 11 Theileria annulata stabilates in bovine PBM ranged from 10(-2.6) to 10(-4.2) dilutions of 1 tick equivalent (t.e.) ml(-1), one stabilate of Theileria parva 10(-2.2)t.e.ml(-1); and three Theileria lestoquardi stabilates in ovine PBM, from 10(-1.5) to 10(-1.8)t.e.ml(-1). Two of the T. annulata stabilates had been used individually to infect groups of calves: stabilate 52 produced more severe disease responses than stabilate 67, as measured by prepatent period, parasitosis, parasitaemia and death or recovery. This corresponded with the sixfold difference found in vitro between the ED50's of these two stabilates. This method is useful not only to measure the infection potential of the sporozoite stabilates but also as an in vitro model for chemotherapeutic and immunological studies of the early stages of theileriosis. |
| Databáze: | OpenAIRE |
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