Identification of the original species of cubilose based on DNA barcode
| Autor: | Jinlin Guo, Feixia Hou, Shasha Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Aerodramus UPGMA original species biology.organism_classification 010603 evolutionary biology 01 natural sciences DNA barcoding cytb Mito Communication 03 medical and health sciences 030104 developmental biology Similarity (network science) Genetic distance Evolutionary biology GenBank Collocalia Genetics Identification (biology) Cubilose Molecular Biology Research Article |
| Zdroj: | Mitochondrial DNA. Part B, Resources article-version (VoR) Version of Record |
| ISSN: | 2380-2359 |
| Popis: | Cubilose, a valuable traditional Chinese medicine, is mainly composed of the saliva by several species of Aerodramus or Collocalia in the Apodidae. Due to rarity, high economic value and huge market demand, its fake or adulteration is frequently found in the market. Therefore, it is urgent to establish a simple and accurate method for authenticating cubilose. DNA barcoding, which is an easy, quick and reliable method, is widely used to trace the origin of traditional Chinese medicine. For identifying the original species of cubilose, cytb gene of 18 cubilose samples including 15 officer cubilose and 3 feather cubilose were amplified and entered into the GenBank database using the BLAST search tool. The genetic distances among 18 cubilose samples were calculated based on the Kimura two parameter (K2P) model. To construct the reference database, 18 cytb sequences of Aerodramus or Collocalia were downloaded from GenBank. The neighbor-joining (NJ) and unweighted pair group method with arithmetic average (UPGMA) trees were constructed based on sequences from GenBank and our dataset. Blast analysis showed that all cubilose samples had the highest similarity with A. fuciphagus, and the sequence similarity reached over 99%. Genetic distance of 18 cubilose samples ranged from 0.000–0.010. Trees constructed by NJ and UPGMA gave similar topology: all cubilose samples clustered together with A. fuciphagus. These result demonstrated that the original species of all 18 cubilose samples were A. fuciphagus, and cytb gene is a good candidate for identifying cubilose. |
| Databáze: | OpenAIRE |
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