Expression of matrix metalloproteinase-2 and -9 and of tissue inhibitor of matrix metalloproteinase-1 in liver regeneration from oval cells in rat
| Autor: | P. Mavier, Dominique Couchie, Y. Laperche, Elie-Serge Zafrani, N. Martin-Garcia, T. Pham Van |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Cellular differentiation Oncostatin M Matrix metalloproteinase Gene Expression Regulation Enzymologic Internal medicine Precursor cell medicine Animals Molecular Biology Cells Cultured In Situ Hybridization Basement membrane Tissue Inhibitor of Metalloproteinase-1 biology Oncostatin M receptor Receptors Oncostatin M Liver regeneration Rats Inbred F344 Cell biology Liver Regeneration Rats Endocrinology medicine.anatomical_structure Liver Matrix Metalloproteinase 9 Hepatocyte biology.protein Hepatocytes Matrix Metalloproteinase 2 |
| Zdroj: | Matrix biology : journal of the International Society for Matrix Biology. 27(8) |
| ISSN: | 0945-053X |
| Popis: | Oval cells participate in liver regeneration when hepatocyte replication is impaired. These precursor cells proliferate in periportal regions and organize in ductules. They are surrounded by a basement membrane, the degradation of which by matrix metalloproteinases (MMP) might trigger their terminal differentiation into hepatocytes. We studied the expression of MMP-2 and MMP-9 and that of one of their tissue inhibitors (TIMP-1) in a model of hepatic regeneration from precursor cells. Regeneration was induced by treating rats with 2-acetylaminofluorene followed by partial hepatectomy. MMP-2 and MMP-9 hepatic expression paralleled oval cell number with a peak at day 9-14 after hepatectomy. They were mainly detected in oval cells. TIMP-1 mRNA and oncostatin M receptor mRNA, a major regulator of TIMP-1 synthesis, markedly increased from day 1 after surgery until day 9 and then declined; they were mainly detected in interlobular bile duct cells and oval cells until day 14. In agreement with the in vivo data, the WB-F344 liver precursor cell line expressed MMP-2 and MMP-9, as well as TIMP-1 and oncostatin M receptor. These data suggest that (a) early increased TIMP-1 synthesis by biliary and oval cells favors basement membrane deposition around proliferating ductular structures through MMP inhibition, (b) delayed increased MMP expression, concomitant to decreased TIMP-1 synthesis, leads to basement membrane degradation, preceding oval cell differentiation, (c) the oncostatin M pathway might play a major role in increased TIMP-1 synthesis. |
| Databáze: | OpenAIRE |
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