In mixed biofilms Enterococcus faecalis benefits from a calcium hydroxide challenge and culturing
| Autor: | J. J. de Soet, Wim Crielaard, Thomas Connert, S. V. van der Waal |
|---|---|
| Přispěvatelé: | Preventive Dentistry, Endodontology, Preventieve tandheelkunde (OII, ACTA), Endodontologie (OII, ACTA) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Root canal Population medicine.disease_cause Enterococcus faecalis Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine education General Dentistry education.field_of_study Calcium hydroxide biology Pseudomonas aeruginosa Chlorhexidine Biofilm 030206 dentistry biology.organism_classification 030104 developmental biology medicine.anatomical_structure chemistry Sodium hypochlorite SDG 6 - Clean Water and Sanitation medicine.drug |
| Zdroj: | van der Waal, S V, Connert, T, Crielaard, W & de Soet, J J 2016, ' In mixed biofilms Enterococcus faecalis benefits from a calcium hydroxide challenge and culturing ', International Endodontic Journal, vol. 49, no. 9, pp. 865-873 . https://doi.org/10.1111/iej.12542 International Endodontic Journal, 49(9), 865-873. Wiley-Blackwell |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/iej.12542 |
| Popis: | AimTo evaluate the fate of Enterococcus faecalis in dual‐species and multispecies biofilms after treatment with calcium hydroxide (Ca(OH)2). MethodologyBiofilms were cultured from laboratory strains of E. faecalis and Pseudomonas aeruginosa, or from microbiota retrieved from primary root canal infections. The biofilms were then treated with Ca(OH)2. The proportion of E. faecalis and P. aeruginosa and their susceptibility to disinfection were evaluated in a viability assay. In the mixed‐species assay, the presence and proportions of E. faecalis before and after Ca(OH)2 treatment were evaluated with a quantitative polymerase chain reaction assay. Groups were compared using Mann–Whitney U‐test and Student's t‐tests. An α ResultsAfter Ca(OH)2 treatment in dual‐species biofilms, the proportion of E. faecalis had increased (P < 0.001), whereas the total number of CFUs per biofilm was equal. Enterococcus faecalis was equally susceptible to disinfection by sodium hypochlorite (NaOCl) or by chlorhexidine. Pseudomonas aeruginosa had become more susceptible to NaOCl disinfection. The root canal isolates contained no detectable amounts of E. faecalis. After biofilm culturing or Ca(OH)2 treatment, it appeared that E. faecalis must have been present in 5 of 6 (83%) root canal samples. ConclusionsCalcium hydroxide favours the population of E. faecalis in a dual‐species biofilm. Culturing multispecies root canal isolates makes E. faecalis detectable. E. faecalis was often present in primary endodontic infections, albeit in low numbers. |
| Databáze: | OpenAIRE |
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