Development and validation an LC-MS/MS method to quantify (+)-borneol in rat plasma: Application to a pharmacokinetic study
| Autor: | Rong Chen, Lu-Ning Sun, Jian Ren, Shi-Bao Yang, Zhen-Yu Miao, Yong-Qing Wang, Chang-Liang Hu, Zheng-Ping Zhang |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Analyte Clinical Biochemistry Administration Oral Biological Availability Atmospheric-pressure chemical ionization Mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Sensitivity and Specificity Analytical Chemistry Borneol Sublingual administration 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics Tandem Mass Spectrometry Animals Chromatography Camphanes Chemistry 010401 analytical chemistry Selected reaction monitoring Reproducibility of Results Cell Biology General Medicine 0104 chemical sciences Bioavailability Rats Linear Models Female Chromatography Liquid |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1109 |
| ISSN: | 1873-376X |
| Popis: | (+)-Borneol, a bicyclic monoterpene, has been shown to possess valuable biological properties and potential as a pharmaceutical agent due to anti-inflammatory, anti-oxidant and GABA receptor-enhancing functions; it also enhances the permeability of the blood brain barrier to improve the efficacy of CNS drugs. In this study, we have developed a simple, selective, and rapid liquid chromatography-tandem mass spectrometry method for the assay of (+)-borneol in rat plasma. Verapamil was used as an internal standard. Plasma samples were deproteinized using methanol. The analyte was detected by a mass spectrometer with positive atmospheric pressure chemical ionization by multiple reaction monitoring mode for transitions at m/z [M + H]+ 137.2 → 81.0 for (+)-borneol and 455.2 → 165.1 for verapamil. The method has been fully validated to ensure good selectivity, a satisfactory lower limit of quantification at 10.0 ng/mL, acceptable intra- and inter-day accuracy, and high precision. The method was used for the pharmacokinetic evaluation of (+)-borneol in Sprague-Dawley rats after intravenous, oral, and sublingual administration. The results indicate that oral bioavailability of (+)-borneol was extremely low but sublingual administration yielded rapid absorption and favorable bioavailability of (+)-borneol. |
| Databáze: | OpenAIRE |
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