The effect of surgical treatment following peritoneal sepsis on hepatic gene expression
| Autor: | Roderick A. Barke, Linda J. Brady, Rebecca B. Chapin, Sabita Roy, Scott Birklid, Paul S. Brady |
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| Rok vydání: | 1996 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Pathology Time Factors endocrine system diseases Gene Expression Mitochondria Liver Mitochondrion Biology Peritoneal Diseases Sepsis Rats Sprague-Dawley Downregulation and upregulation Internal medicine Gene expression medicine Animals heterocyclic compounds Carnitine Carnitine O-palmitoyltransferase RNA Messenger neoplasms Gene Messenger RNA Carnitine O-Palmitoyltransferase medicine.disease digestive system diseases Rats Transcription Factor AP-1 Endocrinology Liver Surgery Proto-Oncogene Proteins c-fos medicine.drug |
| Zdroj: | The Journal of surgical research. 60(1) |
| ISSN: | 0022-4804 |
| Popis: | Peritoneal sepsis results in downregulation of the gene that codes for the hepatic mitochondrial enzyme carnitine palmitoyltransferase (CPT). The inhibition of hepatic CPT transcription by sepsis is thought to be mediated, in part, by increased expression of the leucine-zipper DNA transcription factor c-fos. In a cecal ligation and puncture (CLP) model, we examined the temporal effect of surgical treatment (cecal excision) on sepsis-induced inhibition of CPT gene expression. We investigated the hypothesis that Fos protein level will inversely correlate with the regulation of CPT gene expression. Specifically, we studied hepatic Fos nucleoprotein accumulation and CPT gene expression as measured by total mitochondrial CPT activity, CPT protein, and CPT mRNA. We investigated the following groups: (i) CLP followed by cecal excision 6, 12, or 24 hr following initial insult, (ii) concurrent CLP control group, and (iii) concurrent sham CLP reference group. When measured 48 hr following initial surgical insult, we conclude that: (i) in the absence of surgical treatment, peritoneal contamination results in a decrease in hepatic CPT gene expression and an increase in Fos nucleoprotein accumulation; (ii) surgical treatment at 6 or 12 hr following initial insult prevents the downregulation in hepatic CPT gene expression and does not result in Fos nucleoprotein accumulation; and (iii) surgical treatment at 24 hr following insult did not prevent the downregulation of hepatic CPT gene expression and results in an increase in hepatic Fos nucleoprotein accumulation. These data are consistent with the hypothesis that sepsis-induced regulation of hepatic c-fos gene expression, in part, is responsible for the downregulation of CPT gene expression. |
| Databáze: | OpenAIRE |
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