Additional file 14: Figure S9. of Knockout of Vdac1 activates hypoxia-inducible factor through reactive oxygen species generation and induces tumor growth by promoting metabolic reprogramming and inflammation

Autor: M. Brahimi-Horn, Giuliano, Sandy, Saland, Estelle, Lacas-Gervais, Sandra, Sheiko, Tatiana, Pelletier, Joffrey, Bourget, Isabelle, Bost, Frédéric, Féral, Chloé, Boulter, Etienne, Tauc, Michel, Ivan, Mircea, Garmy-Susini, Barbara, Popa, Alexandra, Mari, Bernard, Jean-Emmanuel Sarry, Craigen, William, Pouysségur, Jacques, Mazure, Nathalie
Rok vydání: 2015
Předmět:
DOI: 10.6084/m9.figshare.c.3646397_d10.v1
Popis: Autophagy was detected in Vdac1 −/− MEF. (A) Representative images of electron micrographs of Wt and Vdac1 −/− MEF incubated in normoxia (Nx) for 24 h. Vdac1 −/− MEF showed autophagosomes with double membranes (arrow). (B) Wt and Vdac1 −/− MEF lysates were analyzed in the absence (−) or presence of chloroquine (CQ) by immunoblotting to LC3. ARD1 was used as a loading control. The ratio of LC3-II/ARD1 was measured.
Databáze: OpenAIRE