Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals
| Autor: | Tania Raymundo, Rosa Paulina Calvillo-Medina, Carlos Adolfo Müller-Morales, Efraín Escudero-Leyva, Diana Gabriela Ponce-Angulo, Juan Campos Guillén, Victor Manuel Bautista-de Lucio |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antifungal Agents Corneal Infection Itraconazole Antifugal susceptibility 030106 microbiology Keratomycosis Microbial Sensitivity Tests Dermatology Infectious Keratitis Biology Deep fungal infection Keratitis Microbiology Cornea Mice 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Drug Resistance Fungal Amphotericin B medicine Animals Humans Fungal keratitis DNA Fungal Mycological Typing Techniques Mexico Phylogeny Aged Voriconazole Mice Inbred BALB C General Medicine medicine.disease corneal ulcer New species Infectious Diseases Mycoses Hypocreales Female Murine model medicine.drug |
| Zdroj: | Mycoses, vol.64, pp.162-173 Kérwá Universidad de Costa Rica instacron:UCR |
| ISSN: | 1439-0507 0933-7407 |
| Popis: | Background Infectious keratitis is the main cause of preventable blindness worldwide, with about 1.5–2.0 million new cases occurring per year. This inflammatory response may be due to infections caused by bacteria, fungi, viruses or parasites. Fungal keratitis is a poorly studied health problem. Objectives This study aimed to identify a new fungal species by molecular methods and to explore the possible efficacy of the three most common antifungals used in human keratitis in Mexico by performing in vitro analysis. The capacity of this pathogen to cause corneal infection in a murine model was also evaluated. Methods The fungal strain was isolated from a patient with a corneal ulcer. To identify the fungus, taxonomic and phylogenetic analyses (nrDNA ITS and LSU data set) were performed. An antifungal susceptibility assay for amphotericin B, itraconazole and voriconazole was carried out. The fungal isolate was used to develop a keratitis model in BALB/c mice; entire eyes and ocular tissues were preserved and processed for histopathologic examination. Results and conclusion This fungal genus has hitherto not been reported with human keratitis in Mexico. We described a new species Purpurecillium roseum isolated from corneal infection. P roseum showed resistance to amphotericin B and itraconazole and was sensitive to voriconazole. In vivo study demonstrated that P roseum had capacity to developed corneal infection and to penetrate deeper corneal tissue. The global change in fungal infections has emphasised the need to develop better diagnostic mycology laboratories and to recognise the group of potential fungal pathogens. UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA) |
| Databáze: | OpenAIRE |
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