Constraining G1-specific transcription to late G1 phase: the MBF-associated corepressor Nrm1 acts via negative feedback
Autor: | Robertus A.M. de Bruin, Paul Russell, Curt Wittenberg, Charly Chahwan, Tatyana I. Kalashnikova, James A. Wohlschlegel, John R. Yates, W. Hayes McDonald |
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Rok vydání: | 2006 |
Předmět: |
Saccharomyces cerevisiae Proteins
Transcription Genetic Saccharomyces cerevisiae Genes Fungal Molecular Sequence Data Cell Cycle Proteins Protein Serine-Threonine Kinases chemistry.chemical_compound Transcription (biology) Gene Expression Regulation Fungal Gene expression Schizosaccharomyces Hydroxyurea Amino Acid Sequence RNA Messenger Promoter Regions Genetic Molecular Biology Gene Feedback Physiological biology Models Genetic G1 Phase Intracellular Signaling Peptides and Proteins Promoter Cell Biology Cell cycle biology.organism_classification Molecular biology Repressor Proteins Checkpoint Kinase 2 chemistry Mutation Trans-Activators Corepressor DNA circulatory and respiratory physiology Protein Binding Transcription Factors |
Zdroj: | Molecular cell. 23(4) |
ISSN: | 1097-2765 |
Popis: | Summary G1-specific transcription in yeast depends upon SBF and MBF. We have identified Nrm1 ( n egative r egulator of M BF targets 1), as a stable component of MBF. NRM1 ( YNR009w ), an MBF-regulated gene expressed during late G1 phase, associates with G1-specific promoters via MBF. Transcriptional repression upon exit from G1 phase requires both Nrm1 and MBF. Inactivation of Nrm1 results in prolonged expression of MBF-regulated transcripts and leads to hydroxyurea (HU) resistance and enhanced bypass of rad53 Δ- and mec1 Δ-associated lethality. Constitutive expression of a stabilized form of Nrm1 represses MBF targets and leads to HU sensitivity. The fission yeast homolog SpNrm1, encoded by the MBF target gene nrm1 + ( SPBC16A3.07c ), binds to MBF target genes and acts as a corepressor. In both yeasts, MBF represses G1-specific transcription outside of G1 phase. A negative feedback loop involving Nrm1 bound to MBF leads to transcriptional repression as cells exit G1 phase. |
Databáze: | OpenAIRE |
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