Gene expression in IFNß signalling pathway differs between monocytes, CD4 and CD8 T cells from MS patients
| Autor: | Oscar Fernandez, Miguel Guerrero, Cristobalina Mayorga, G. Luque, Begoña Oliver-Martos, Margarita Suardíaz, Teresa Órpez, Juan Antonio García-León, R. Maldonado-Sanchez, M.J. Pinto-Medel, Laura Leyva |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Multiple Sclerosis Immunology Gene Expression Receptor Interferon alpha-beta CD8-Positive T-Lymphocytes Monocytes Gene expression Humans Immunology and Allergy Cytotoxic T cell STAT1 STAT2 biology Reverse Transcriptase Polymerase Chain Reaction JAK-STAT signaling pathway Interferon-beta Real-time polymerase chain reaction Neurology Tyrosine kinase 2 biology.protein Cancer research Female Neurology (clinical) CD8 Signal Transduction |
| Zdroj: | Journal of Neuroimmunology. 230:153-159 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/j.jneuroim.2010.10.033 |
| Popis: | IFNß exerts its activity through the interaction with IFNAR, through activation of the JAK/STAT pathway. We analyzed the changes in IFNAR1, IFNAR2, STAT1, STAT2, Tyk2, JAK1, IRF9 and MxA gene expressions after prolonged IFNß treatment, in isolated mononuclear-cell subpopulations from MS patients, by real time PCR. The effect of IFNß on gene expression differed depending on the subpopulation assessed. The data suggest that CD8+ T cells are the most influenced by prolonged IFNß therapy as IFNAR2, Tyk2, IRF9 and Jak1 expressions were decreased, whereas MxA expression was increased in these cells. |
| Databáze: | OpenAIRE |
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