Gegen Qinlian Decoction abates nonalcoholic steatohepatitis associated liver injuries via anti-oxidative stress and anti-inflammatory response involved inhibition of toll-like receptor 4 signaling pathways
Autor: | Cao Zheng, Xiao-quan Luo, Ke-zhong Deng, Li-fen Zhou, Xiao Qin, Ya-nan Xue, Chen Chen, Min Shi, Ying-qian Cao, Jun-qing Sheng, Tong-tong Liu, Chang-hua Zhang |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Lipopolysaccharides Proteomics Lipopolysaccharide Anti-Inflammatory Agents Pharmacology medicine.disease_cause Antioxidants chemistry.chemical_compound Mice 0302 clinical medicine Non-alcoholic Fatty Liver Disease Non-alcoholic steatohepatitis Toll-like receptor Chemistry General Medicine 030220 oncology & carcinogenesis GNMT Cytokines lipids (amino acids peptides and proteins) Female medicine.symptom Signal transduction Inflammation Mediators Signal Transduction Inflammation RM1-950 Models Biological Cell Line 03 medical and health sciences medicine Animals Gegen Qinlian Decoction Gene Expression Profiling nutritional and metabolic diseases Lipid metabolism Lipid Metabolism Rats Toll-Like Receptor 4 Disease Models Animal Oxidative Stress Hepatic lipid metabolism 030104 developmental biology TLR4 Therapeutics. Pharmacology Transcriptome Oxidative stress Biomarkers Drugs Chinese Herbal |
Zdroj: | Biomedicine & Pharmacotherapy, Vol 126, Iss, Pp 110076-(2020) |
ISSN: | 1950-6007 |
Popis: | Gegen Qilian Decoction (GGQLD) is a well-established classic Chinese medicine prescription in treating nonalcoholic steatohepatitis (NASH). However, the molecular mechanism of GGQLD action on NASH is still not clear. This study aimed to assess the anti-NASH effect of GGQLD, and to explore its molecular mechanisms in vivo and in vitro. In HFD-fed rats, GGQLD decreased significantly serum triglyceride (TG), cholesterol (CHO), total bile acid (TBA), low-density lipoprotein (LDL), free fatty acid (FFA) and lipopolysaccharide (LPS) levels, increased levels of differentially expressed proteins (DEPs) Ahcy, Gpx1, Mat1a, GNMT, and reduced the expression of ALDOB. In RAW264.7 macrophages, GGQLD reduced the expression levels of inflammatory factors TNF-α and IL-6 mRNA, and diminished NASH by increasing differentially expressed genes (DEGs) CBS, Mat1a, Hnf4α and Pparα to reduce oxidative stress or lipid metabolism. The results of DEGs verification also showed that GGQLD up-regulated expressions of Hnf4α, Pparα and Cbs genes. In HepG2 cells, GGQLD decreased IL-6 levels and intracellular TG content, and inhibited FFA-induced expression of toll-like receptor 4 (TLR4). In summary, GGQLD abates NASH associated liver injuries via anti-oxidative stress and anti-inflammatory response involved inhibition of TLR4 signal pathways. These findings provide new insights into the anti-NASH therapy by GGQLD. |
Databáze: | OpenAIRE |
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