Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
| Autor: | Yan Wang, Stephen J. Powers, Kirsty L. Hassall, Alison K. Huttly, Peter R. Shewry, Caroline A. Sparks, Yongfang Wan, Peter Buchner, Malcolm J. Hawkesford, Doris Rentsch, Jane L. Ward, Zhiqiang Shi |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Magnetic Resonance Spectroscopy Fruit and Seed Anatomy Amino Acid Transport Systems Physiology Overexpression Flour Plant Science 580 Plants (Botany) Biochemistry 01 natural sciences Endosperm RNA interference Glutenin Gene Expression Regulation Plant Aleurone Medicine and Health Sciences RNA-Seq Amino Acids Promoter Regions Genetic Chromatography High Pressure Liquid Triticum Plant Proteins chemistry.chemical_classification Multidisciplinary biology Chemistry Amino acid transporter Plant Anatomy Eukaryota food and beverages Plants Grain size Up-Regulation Amino acid Nucleic acids Genetic interference Physiological Parameters Wheat Amino Acid Analysis Medicine Hyperexpression Techniques Epigenetics Grain metabolites Research Article Glutens Amino Acid Transport Systems Acidic Nitrogen Science Phloem Real-Time Polymerase Chain Reaction Research and Analysis Methods 03 medical and health sciences Genetics Gene Expression and Vector Techniques Storage protein Grasses Molecular Biology Techniques Wheat grain Molecular Biology Nutrition Molecular Biology Assays and Analysis Techniques Body Weight fungi Organisms Fungi Biology and Life Sciences Grain nitrogen Yeast Diet Plant Leaves Glutamine 030104 developmental biology Food RNAi Glycine biology.protein RNA Gene expression Edible Grain 010606 plant biology & botany |
| Zdroj: | Wan, Yongfang; Wang, Yan; Shi, Zhiqiang; Rentsch, Doris; Ward, Jane L.; Hassall, Kirsty; Sparks, Caroline A.; Huttly, Alison K.; Buchner, Peter; Powers, Stephen; Shewry, Peter R.; Hawkesford, Malcolm J. (2021). Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain. PLoS ONE, 16(2), e0246763. Public Library of Science 10.1371/journal.pone.0246763 PLoS ONE PLoS ONE, Vol 16, Iss 2, p e0246763 (2021) |
| DOI: | 10.1371/journal.pone.0246763 |
| Popis: | Amino acids are delivered into developing wheat grains to support the accumulation of storage proteins in the starchy endosperm, and transporters play important roles in regulating this process. RNA-seq, RT-qPCR, and promoter-GUS assays showed that three amino acid transporters are differentially expressed in the endosperm transfer cells (TaAAP2), starchy endosperm cells (TaAAP13), and aleurone cells and embryo of the developing grain (TaAAP21), respectively. Yeast complementation revealed that all three transporters can transport a broad spectrum of amino acids. RNAi-mediated suppression of TaAAP13 expression in the starchy endosperm did not reduce the total nitrogen content of the whole grain, but significantly altered the composition and distribution of metabolites in the starchy endosperm, with increasing concentrations of some amino acids (notably glutamine and glycine) from the outer to inner starchy endosperm cells compared with wild type. Overexpression of TaAAP13 under the endosperm-specific HMW-GS (high molecular weight glutenin subunit) promoter significantly increased grain size, grain nitrogen concentration, and thousand grain weight, indicating that the sink strength for nitrogen transport was increased by manipulation of amino acid transporters. However, the total grain number was reduced, suggesting that source nitrogen remobilized from leaves is a limiting factor for productivity. Therefore, simultaneously increasing loading of amino acids into the phloem and delivery to the spike would be required to increase protein content while maintaining grain yield. © 2021 Wan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Databáze: | OpenAIRE |
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