Surface Modification with NGF-Loaded Chitosan/Heparin Nanoparticles for Improving Biocompatibility of Cardiovascular Stent
| Autor: | Ye Ye, Shihui Liu, Yangzheng Li, Tao Liu, Hai-xin Song, Xiaotian Yang, Tao Wu, Jian-hua Li, Bo Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Article Subject Endothelium Biocompatibility 02 engineering and technology Endothelial progenitor cell Chitosan 03 medical and health sciences chemistry.chemical_compound medicine Internal medicine Molecular Biology Regeneration (biology) Cell Biology Heparin 021001 nanoscience & nanotechnology RC31-1245 Endothelial stem cell 030104 developmental biology medicine.anatomical_structure chemistry Biophysics Surface modification 0210 nano-technology Research Article medicine.drug |
| Zdroj: | Stem Cells International Stem Cells International, Vol 2021 (2021) |
| ISSN: | 1687-9678 1687-966X |
| DOI: | 10.1155/2021/9941143 |
| Popis: | Late thrombosis and restenosis remain major challenges to the safety of drug-eluting stents. Biofunctional modification to endow the surface with selective anticoagulation and promote endothelium regeneration has become a hotpot recently. In this study, chitosan and heparin were found to form three-dimensional nanoparticles by spontaneous electrostatic interaction. Based on the specific binding properties between heparin and nerve growth factor (NGF), a new type of NGF-loaded heparin/chitosan nanoparticles was constructed for surface modification. The results of material characterization show that the nanoparticles are successfully immobilized on the surface of the material. In vitro blood compatibility and endothelial cell compatibility assay showed that the modified surface could selectively inhibit platelet adhesion and smooth muscle cell overproliferation, while accelerating endothelialization via promoting endothelial cell proliferation and enhancing endothelial progenitor cell mobilization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|