Comparative genomics of the major agents of human and animal Sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis

Autor: Marcus de Melo Teixeira, Ana Karina Rodrigues Abadio, Héctor Villalobos-Duno, Fabiana Freire Mendes de Oliveira, Amanda L N Barros, Luiz Gonzaga Paula de Almeida, Jeronimo C. Ruiz, Marjorie Mendes Marini, Ana Tereza Ribeiro de Vasconcelos, Sandro Rogério de Almeida, Oberdan de Lima Cunha, Bernard Henrissat, Patrícia Pereira Alves da Silva, Sybren de Hoog, Patrícia S Cisalpino, Leila M. Lopes-Bezerra, Patrícia Albuquerque, Alan Viggiano Neto, José Franco da Silveira, Tayná C dos Santos, Karen Spadari Ferreira, Paula H. Kubitschek-Barreira, Rangel C. Souza, Marcel M.L. Cunha, Larissa Fernandes, Hugo Costa Paes, Maria Sueli Soares Felipe, André Moraes Nicola, Claudia B Chaucanez, Alexandra L. Gerber, Luciana M. Oliveira, Marco A. Soares, Luisa Defranco Ferreira Peconick, Fernanda L. Alves, Jason E. Stajich, Nathalia C de Andrade, Gustavo Niño-Vega, Vicente de Paulo Martins, Héctor M. Mora-Montes, Erika Seki Kioshima, Lorena da Silveira Derengowski
Přispěvatelé: Evolutionary Biology (IBED, FNWI)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Teixeira, MM; de Almeida, LGP; Kubitschek-Barreira, P; Alves, FL; Kioshima, ÉS; Abadio, AKR; et al.(2014). Comparative genomics of the major fungal agents of human and animal Sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis. BMC Genomics, 15(1), 943. doi: 10.1186/1471-2164-15-943. UC Riverside: Retrieved from: http://www.escholarship.org/uc/item/2jp4s4xm
BMC Genomics
Repositório Institucional da UnB
Universidade de Brasília (UnB)
instacron:UNB
BMC Genomics, 15. BioMed Central
ISSN: 1471-2164
Popis: Background The fungal genus Sporothrix includes at least four human pathogenic species. One of these species, S. brasiliensis, is the causal agent of a major ongoing zoonotic outbreak of sporotrichosis in Brazil. Elsewhere, sapronoses are caused by S. schenckii and S. globosa. The major aims on this comparative genomic study are: 1) to explore the presence of virulence factors in S. schenckii and S. brasiliensis; 2) to compare S. brasiliensis, which is cat-transmitted and infects both humans and cats with S. schenckii, mainly a human pathogen; 3) to compare these two species to other human pathogens (Onygenales) with similar thermo-dimorphic behavior and to other plant-associated Sordariomycetes. Results The genomes of S. schenckii and S. brasiliensis were pyrosequenced to 17x and 20x coverage comprising a total of 32.3 Mb and 33.2 Mb, respectively. Pair-wise genome alignments revealed that the two species are highly syntenic showing 97.5% average sequence identity. Phylogenomic analysis reveals that both species diverged about 3.8-4.9 MYA suggesting a recent event of speciation. Transposable elements comprise respectively 0.34% and 0.62% of the S. schenckii and S. brasiliensis genomes and expansions of Gypsy-like elements was observed reflecting the accumulation of repetitive elements in the S. brasiliensis genome. Mitochondrial genomic comparisons showed the presence of group-I intron encoding homing endonucleases (HE’s) exclusively in S. brasiliensis. Analysis of protein family expansions and contractions in the Sporothrix lineage revealed expansion of LysM domain-containing proteins, small GTPases, PKS type1 and leucin-rich proteins. In contrast, a lack of polysaccharide lyase genes that are associated with decay of plants was observed when compared to other Sordariomycetes and dimorphic fungal pathogens, suggesting evolutionary adaptations from a plant pathogenic or saprobic to an animal pathogenic life style. Conclusions Comparative genomic data suggest a unique ecological shift in the Sporothrix lineage from plant-association to mammalian parasitism, which contributes to the understanding of how environmental interactions may shape fungal virulence. . Moreover, the striking differences found in comparison with other dimorphic fungi revealed that dimorphism in these close relatives of plant-associated Sordariomycetes is a case of convergent evolution, stressing the importance of this morphogenetic change in fungal pathogenesis. Electronic supplementary material The online version of this article (doi:10.1186/1471-2164-15-943) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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