Association of hormone-sensitive lipase (HSL) gene polymorphisms with the intramuscular fat content in two Chinese beef cattle breeds
| Autor: | Hamed Shoorei, Ayman H. Abd El-Aziz, Linsheng Gui, Sayed Haidar Abbas Raza, Sameeullah Memon, Ali Raza Jahejo, Zhou Li, Irfan Ullah, Rajwali Khan, Gui Yao Liu, Guobo Quan |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Candidate gene Population Hormone-sensitive lipase Beef cattle Biology 01 natural sciences Genetic analysis Polymorphism Single Nucleotide Linkage Disequilibrium Andrology 03 medical and health sciences Exon Species Specificity Genetics Animals Amino Acid Sequence education Muscle Skeletal Genetic Association Studies 030304 developmental biology 0303 health sciences education.field_of_study food and beverages Sterol Esterase Red Meat Adipose Tissue Haplotypes Genetic marker Cattle Intramuscular fat 010606 plant biology & botany |
| Zdroj: | Genomics. 112(6) |
| ISSN: | 1089-8646 |
| Popis: | Hormone-sensitive lipase (HSL) was considered as an essential enzyme in glucolipid metabolism. It has been proposed to be a lead candidate gene for genetic markers of lipid deposition in livestock. The aim of this study was to identify sequence variants (SVs) of the bovine HSL gene and evaluate the relations to intramuscular fat in two indigenous Chinese beef cattle breeds. Expression analysis by quantitative real-time polymerase chain reactions (qPCR) indicated that expression levels of bovine HSL gene were highest in the perirenal fat and heart within two different age stage (adult and calf), respectively. Five SVs were identified by direct DNA sequencing, which included four missense mutations (g.16563C>T, g.16734G>A, g.16896A>G, g.17388G>T) in exon 8 and a synonymous mutation (g.17402C>T) in exon 9. Population genetic analysis showed that except for g.16563C>T and g.17402C>T, all the other detected SVs strongly affected the bovine intramuscular fat content (P |
| Databáze: | OpenAIRE |
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