An ungrouped plant kinesin accumulates at the preprophase band in a cell cycle-dependent manner
| Autor: | Richard J. Cyr, Jennelle L. Malcos |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Green Fluorescent Proteins
Molecular Sequence Data Arabidopsis Kinesins Mitosis Biology Microtubules Prophase Motor protein Structural Biology Microtubule Amino Acid Sequence Phylogeny Sequence Homology Amino Acid Arabidopsis Proteins Cell Cycle Computational Biology Cell Biology biology.organism_classification Cell biology Mutagenesis Armadillo repeats Mutation Preprophase band Kinesin |
| Zdroj: | Cytoskeleton. 68:247-258 |
| ISSN: | 1949-3584 |
| DOI: | 10.1002/cm.20508 |
| Popis: | Past phylogenic studies have identified a plant-specific, ungrouped family of kinesins in which the motor domain does not group to one of the fourteen recognized families. Members of this family contain an N-terminal motor domain, a C-terminal armadillo repeat domain and a conserved destruction box (D-BOX) motif. This domain architecture is unique to plants and to a subset of protists. Further characterization of one representative member from Arabidopsis, Arabidopsis thaliana KINESIN ungrouped clade, gene A (AtKINUa), was completed to ascertain its functional role in plants. Fluorescence confocal microscopy revealed an accumulation of ATKINUA:GFP at the preprophase band (PPB) in a cell cycle-dependent manner in Arabidopsis epidermal cells and tobacco BY-2 cells. Fluorescence accumulation was highest during prophase and decreased after nuclear envelope breakdown. A conserved D-BOX motif was identified through alignment of AtKINU homologous sequences. Mutagenesis work with D-BOX revealed that conserved residues were necessary for the observed degradation pattern of ATKINUA:GFP, as well as the targeted accumulation at the PPB. Overall results suggest that AtKINUa is necessary for normal plant growth and/or development and is likely involved with PPB organization through microtubule association and specific cell cycle regulation. The D-BOX motif may function to bridge microtubule organization with changes that occur during progression through mitosis and may represent a novel regulatory motif in plant microtubule motor proteins. |
| Databáze: | OpenAIRE |
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