MK-801-induced behavioral and dopaminergic responses in the shell part of the nucleus accumbens in adult male rats are disrupted after neonatal blockade of the ventral subiculum

Autor: Elora Kereselidze, Hana Saoud, Alain Louilot, Séverine Eybrard
Přispěvatelé: UMR-S U1114, Faculty of Medicine-ULP
Rok vydání: 2021
Předmět:
Male
medicine.medical_specialty
In vivo voltammetry
Dopamine
Context (language use)
Nucleus accumbens
Hippocampus
Receptors
N-Methyl-D-Aspartate
Nucleus Accumbens
Rats
Sprague-Dawley
03 medical and health sciences
Cellular and Molecular Neuroscience
chemistry.chemical_compound
0302 clinical medicine
Postsynaptic potential
TTX postnatal Inactivation
Internal medicine
medicine
Animals
[SDV.BDD]Life Sciences [q-bio]/Development Biology
030304 developmental biology
0303 health sciences
Dose-Response Relationship
Drug
Dopaminergic Neurons
Dopaminergic
Age Factors
Subiculum
Cell Biology
Animal modeling
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
Rats
MK-801 administration
Endocrinology
Animals
Newborn
nervous system
chemistry
Schizophrenia
Tetrodotoxin
NMDA receptor
Female
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Dizocilpine Maleate
Excitatory Amino Acid Antagonists
Locomotion
030217 neurology & neurosurgery
medicine.drug
Zdroj: Neurochemistry International
Neurochemistry International, Elsevier, 2021, Volume 150, pp.105195. ⟨10.1016/j.neuint.2021.105195⟩
ISSN: 0197-0186
DOI: 10.1016/j.neuint.2021.105195
Popis: International audience; The present study was conducted in the context of animal modeling of schizophrenia. It investigated in adult male rats, after transient neonatal blockade of the ventral subiculum (VSub), the impact of a very specific non-competitive antagonist of NMDA receptors (MK-801) on locomotor activity and dopaminergic (DAergic) responses in the dorsomedial shell part of the nucleus accumbens (Nacc), a striatal subregion described as the common target region for antipsychotics.The functional neonatal inactivation of the VSub was achieved by local microinjection of tetrodotoxin (TTX) at postnatal day 8 (PND8). Control pups were microinjected with the solvent phosphate buffered saline (PBS). Locomotor responses and DAergic variations in the dorsomedial shell part of the Nacc were measured simultaneously using in vivo voltammetry in awake, freely moving male animals after sc administration of MK-801. The following results were obtained: 1) a dose-dependent increase in locomotor activity in PBS and TTX animals, greater in TTX rats/PBS rats; and 2) divergent DAergic responses for PBS and TTX animals. A decrease in DA levels with a return to around basal values was observed in PBS animals. An increase in DA levels was obtained in TTX animals. The present data suggest that neonatal blockade of the VSub results in disruption in NMDA glutamatergic transmission, causing a disturbance in DA release in the dorsomedial shell in adults male rats. In the context of animal modeling of schizophrenia using the same approach it would be interesting to investigate possible changes in postsynaptic NMDA receptors-related proteins in the dorsomedial shell region in the Nacc.
Databáze: OpenAIRE
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