Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer
| Autor: | Paul M. Radford, R. Elizabeth Sockett, Jamie Twycross, Mathew Diggle, Gemma Clark, David Negus, Christopher I. Moore, Dhaarini Raghunathan, Michelle Baker, Jess Tyson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
Serum 0301 basic medicine Bdellovibrio Pathogen Serum Klebsiella modelling Klebsiella pneumoniae Science 030106 microbiology Human pathogen Buffers Models Biological Article Predation Microbiology 03 medical and health sciences Blood serum Antibiosis Humans Natural enemies Bacteriological Techniques Microbial Viability Multidisciplinary biology Bdellovibrio bacteriovorus biology.organism_classification Bacterial Load Culture Media Microscopy Fluorescence Medicine Algorithms Serum chemistry Beta lactam antibiotics |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-18 (2017) Scientific Reports |
| ISSN: | 2381-3652 2045-2322 |
| Popis: | In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains and so B. bacteriovorus have been proposed as “living antibiotics” to combat antimicrobially-resistant pathogens. Predator-prey interactions are complex and can be altered by environmental components. To be effective B. bacteriovorus predation needs to work in human body fluids such as serum where predation dynamics may differ to that studied in laboratory media. Here we combine mathematical modelling and lab experimentation to investigate the predation of an important carbapenem-resistant human pathogen, Klebsiella pneumoniae, by B. bacteriovorus in human serum versus buffer. We show experimentally that B. bacteriovorus is able to reduce prey numbers in each environment, on different timescales. Our mathematical model captures the underlying dynamics of the experimentation, including an initial predation-delay at the predator-prey-serum interface. Our research shows differences between predation in buffer and serum and highlights both the potential and limitations of B. bacteriovorus acting therapeutically against K. pneumoniae in serum, informing future research into the medicinal behaviours and dosing of this living antibacterial. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|