Ultrasensitive and Simultaneous SERS Detection of Multiplex MicroRNA Using Fractal Gold Nanotags for Early Diagnosis and Prognosis of Hepatocellular Carcinoma
| Autor: | Ping-Hua Sun, Jiamin Wu, Huaihong Cai, Haibo Zhou, Dong Chen, Xia Zhou, Xiaoling Lin, Ping Li, Christoph Haisch, Jinhua Wang, Yun Zheng, Reinhard Niessner, Pengcheng Zhang, Zhengjin Jiang, Ziwei Hu |
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| Rok vydání: | 2021 |
| Předmět: |
Carcinoma
Hepatocellular medicine.medical_treatment Metal Nanoparticles Biosensing Techniques Spectrum Analysis Raman 010402 general chemistry 01 natural sciences Analytical Chemistry Limit of Detection medicine Humans Multiplex Early Detection of Cancer Detection limit Chemistry Liver Neoplasms 010401 analytical chemistry Cancer Prognosis medicine.disease 0104 chemical sciences MicroRNAs Fractals Colloidal gold Hepatocellular carcinoma Cancer research Gold Hepatectomy Liver cancer Biosensor |
| Zdroj: | Analytical Chemistry. 93:8799-8809 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.1c00478 |
| Popis: | Sensitive and simultaneous detection of multiple cancer-related biomarkers in serum is essential for diagnosis, therapy, prognosis, and staging of cancer. Herein, we proposed a magnetically assisted sandwich-type surface-enhanced Raman scattering (SERS)-based biosensor for ultrasensitive and multiplex detection of three hepatocellular carcinoma-related microRNA (miRNA) biomarkers. The biosensor consists of an SERS tag (probe DNA-conjugated DNA-engineered fractal gold nanoparticles, F-AuNPs) and a magnetic capture substrate (capture DNA-conjugated Ag-coated magnetic nanoparticles, AgMNPs). The proposed strategy achieved simultaneous and sensitive detection of three miRNAs (miRNA-122, miRNA-223, and miRNA-21), and the limits of detection of the three miRNAs in human serum are 349 aM for miRNA-122, 374 aM for miRNA-223, and 311 aM for miRNA-21. High selectivity and accuracy of the SERS biosensor were proved by practical analysis in human serum. Moreover, the biosensor exhibited good practicability in multiplex detection of three miRNAs in 92 clinical sera from AFP-negative patients, patients before and after hepatectomy, recurred and relapse-free patients after hepatectomy, and hepatocellular carcinoma patients at distinct Barcelona clinic liver cancer stages. The experiment results demonstrate that our SERS-based assay is a promising candidate in clinical application and exhibited potential for the prediction, diagnosis, monitoring, and staging of cancers. |
| Databáze: | OpenAIRE |
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