Relationship between coronary flow and adenosine release in reactive hyperemia
| Autor: | S. Jamal Mustafa, Michael O. Onwochei |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
medicine.medical_specialty Adenosine Time Factors Guinea Pigs Hyperemia In Vitro Techniques High-performance liquid chromatography General Biochemistry Genetics and Molecular Biology Coronary circulation Internal medicine Coronary Circulation Occlusion Medicine Animals General Pharmacology Toxicology and Pharmaceutics Reactive hyperemia business.industry Myocardium General Medicine Blood flow Perfusion Kinetics medicine.anatomical_structure Endocrinology Anesthesia Liberation business medicine.drug |
| Zdroj: | Life sciences. 36(2) |
| ISSN: | 0024-3205 |
| Popis: | A linear relationship was found between coronary flow and adenosine release during the course of reactive hyperemia. Isolated guinea pig heart was perfused with a modified Krebs Ringer bicarbonate buffer containing 2.0 mM pyruvate. Hyperemia was induced with 30, 60 and 90-second coronary occlusions. The hyperemic response was divided into three consecutive 13-second intervals (I, II and III), and perfusate efflux from coronary circulation was collected during the last 10 seconds of each interval for adenosine assay using the HPLC. The data show a control flow of 3.13 +/- 0.4 ml/min/g and adenosine release of 66 +/- 4 pmoles/min/g. Flow increased by 99, 38 and 23% at I, II and III, respectively following 30-second occlusion, whereas adenosine release increased by 241, 132 and 91% for I, II and III. A 60-second occlusion increased the flow by 125, 64 and 34% with a simultaneous increase in the release of adenosine by 464, 155 and 133%, respectively, for I, II and III. Marked elevations in flow (165, 92 and 59%) and in adenosine release (659, 194 and 176%) for I, II and III were observed following 90-second occlusion. The linear relationship between coronary flow and adenosine release had r values of 0.84, 0.74 and 0.88 for 30, 60 and 90-second occlusions, respectively. This study quantifies the relationship between coronary flow and adenosine release during the course of reactive hyperemia. It also suggests that on a percent basis, adenosine contributes equally to the hyperemia at I, II and III. |
| Databáze: | OpenAIRE |
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