Comparative bioaugmentation with a consortium and a single strain in a phenanthrene-contaminated soil: Impact on the bacterial community and biodegradation
| Autor: | Sabrina Festa, Irma Susana Morelli, Bibiana Marina Coppotelli |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Bioaugmentation Biotecnología del Medio Ambiente Soil Science PYROSEQUENCING INGENIERÍAS Y TECNOLOGÍAS 010501 environmental sciences 01 natural sciences COMMUNITY COMPOSITION Microbiology 03 medical and health sciences chemistry.chemical_compound BIOAUGMENTATION Arthrobacter Food science Microbial inoculant Bioremediación Diagnóstico Biotecnológico en Gestión Medioambiental 0105 earth and related environmental sciences Ecology biology Phenanthrene biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Soil contamination PHENANTHRENE DEGRADATION Burkholderiales 030104 developmental biology Microbial population biology chemistry BACTERIAL CONSORTIUM Microcosm |
| Zdroj: | Applied Soil Ecology. 98:8-19 |
| ISSN: | 0929-1393 |
| DOI: | 10.1016/j.apsoil.2015.08.025 |
| Popis: | The efficiency of two inoculation strategies, using a consortium (CON) or an isolated strain (AM), on phenanthrene-contaminated soil was determined with special concern on the study of the bacterial community composition by PCR-DGGE and pyrosequencing of 16S rRNA gene fragments. Both strategies stimulated the phenanthrene degradation, increasing the cultivable heterotrophic bacteria number and biological activity. At the end of the treatments, the microcosms inoculated with AM reached the lowest values of phenanthrene but also the lowest dehydrogenase activity. In DGGE patterns a reduction in number of bands in the contaminated and inoculated microcosms was observed, being the most significant differences attributed to inoculation with AM. The pyrosequencing technique yielded results that correlated with the fingerprint, showing that the bacterial community composition based on relative abundance was significantly modified by treatments. Sphingomonadales and Burkholderiales were highly stimulated by phenanthrene contamination and inoculation. In the Phe microcosm, the higher increase in Actinomycetales (mainly Arthrobacter) was observed. Effectively, the use of the strain AM as inoculant became the best strategy to remediate the soil mainly based on the degradation efficiency, however it caused more drastic changes in microbial community than inoculation with CON, what can be compromising the ulterior functionality of the soil. Fil: Festa, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Coppotelli, Bibiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Morelli, Irma Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina |
| Databáze: | OpenAIRE |
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