Bone marrow reconstitution of lethally irradiated canines using autologous bone marrow fractions obtained by counterflow centrifugation-elutriation
| Autor: | John F. Jemionek, Thomas J. MacVittie, S. B. Espy, T. J. Contreras, Rodney L. Monroy |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Myeloid Bone Marrow Cells Cell Count Centrifugation Cell Separation Biology Colony-Forming Units Assay Leukocyte Count Dogs Bone Marrow Nucleated cell medicine Animals Platelet Bone Marrow Transplantation Platelet Count Hematology Counterflow centrifugation elutriation Autologous bone medicine.anatomical_structure Female Bone marrow Stem cell Whole-Body Irradiation |
| Zdroj: | British Journal of Haematology. 51:585-594 |
| ISSN: | 1365-2141 0007-1048 |
| DOI: | 10.1111/j.1365-2141.1982.tb02822.x |
| Popis: | Canine bone marrow fractionated by counterflow centrifugation-elutriation results in three areas of nucleated cell recovery. Fraction 1 accounts for 50% of the total nucleated cells and 25-40% of the total recovered CFU-GM activity. Fraction 2 contains less than 2% of the total nucleated cells and less than 0.2% of the CFU-GM activity. Fraction 3 accounts for approximately 50% of the total nucleated cell recovery and 60-75% of the total recovered CFU-GM activity. Animal survival was not directly correlated with the levels of CFU-GM activity in the transfused fractions. Autologous infusion of these fractions into irradiated canines (9 Gy, 0.1 Gy/min) resulted in distinct survival profiles. Canines receiving autologous fraction-2 cells showed no haematological reconstitution, with death occurring on days 10-11 post-irradiation. Canines receiving autologous fraction-3 cells showed limited myeloid repopulation of both the bone marrow and peripheral blood with a mean survival time for 24 d. Canines receiving autologous fraction-1 cells showed complete haematological reconstitution after 48 d and long-term survival. The data may indicate a separation or enrichment of pluripotential stem cells (fraction 1) from committed myeloid progenitor cells (fraction 3). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text