| Autor: |
John K. Grey, Chao Cui, Xiaowei Ma, Brandon Burnside, Kelton A. Schleyer, Benjamin D. Datko, Lina Cui |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| DOI: |
10.1101/2020.01.28.923672 |
| Popis: |
Fluorophores experience altered emission lifetimes when incorporated into and liberated from macromolecules or molecular aggregates; this trend suggests the potential for a fluorescent, responsive probe capable of undergoing self-assembly and aggregation and consequently altering the lifetime of its fluorescent moiety to provide contrast between the active and inactive probes. We developed a cyanobenzothioazole-fluorescein conjugate (1), and spectroscopically examined the lifetime changes caused by its reduction-induced aggregationin vitro. A decrease in lifetime was observed for compound1in a buffered system activated using the biological reducing agent glutathione, suggesting a possible approach for designing responsive self-aggregating lifetime imaging probes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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