Lactobacillus helveticus : Strain Typing and Genome Size Estimation by Pulsed Field Gel Electrophoresis
| Autor: | Annette Rouault, Michel Gautier, Stéphane Guezenec, Sylvie Lortal |
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| Přispěvatelé: | UR 0121 Laboratoire de recherche de Technologie Laitière, Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 1997 |
| Předmět: |
DNA
Bacterial [SDV]Life Sciences [q-bio] Restriction Mapping génétique moléculaire Biology Applied Microbiology and Biotechnology Microbiology différenciation des souches SmaI Restriction fragment Endonuclease Restriction map Pulsed-field gel electrophoresis électrophorèse Gel electrophoresis Genetics Lactobacillus helveticus génome UPGMA food and beverages General Medicine biology.organism_classification Molecular biology Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field lactobacillus biology.protein Genome Bacterial |
| Zdroj: | Current Microbiology (34), 180-185. (1997) Current Microbiology Current Microbiology, Springer Verlag, 1997, 34, pp.180-185 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s002849900165 |
| Popis: | Genomic DNAs of 22 strains of Lactobacillus helveticus of various geographical origins were analyzed by pulsed-field gel electrophoresis. Two endonucleases, SmaI and SgrAI, of the 19 tested produced DNA fragments useful for strain comparison. With the endonuclease SmaI, a characteristic restriction pattern was identified for 18 of the 22 strains. The percentage of similarity (Dice coefficient) between the profiles varied between 26% and 100%, and clustering was accomplished by using the unweighted pair group method with arithmetic averages (UPGMA). For the strains showing identical profiles,the high genomic similarity was confirmed when the endonuclease SgrAI was used instead of SmaI. From summation of SmaI and SgrAI fragments from three L. helveticus strains(CNRZ 241, CNRZ 303, and CIP 57.15), the genomic length was estimated at ca.1. 85-2.0 Mb. |
| Databáze: | OpenAIRE |
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