Structure elucidation, anticancer and antioxidant activities of a novel polysaccharide from Gomphus clavatus Gray
| Autor: | Yiling Hou, Wanru Hou, Fang Wang, Lei Fu, Xiang Ding, Hongqing Zhu, Hang Qin, Yuanxiu Zhu, Panpan Wang, Nan Zhang, Wei Qu |
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| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Magnetic Resonance Spectroscopy Antioxidant Gomphus clavatus medicine.medical_treatment Antineoplastic Agents Polysaccharide Antioxidants Gas Chromatography-Mass Spectrometry Hydrolysis chemistry.chemical_compound Polysaccharides medicine Humans Butylated hydroxytoluene chemistry.chemical_classification biology Galactose Free Radical Scavengers General Medicine Nuclear magnetic resonance spectroscopy biology.organism_classification Housekeeping gene Oncology Biochemistry chemistry Sephadex Oxidation-Reduction |
| Zdroj: | Oncology Reports. 33:3162-3170 |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2015.3921 |
| Popis: | A novel heteropolysaccharide from the fruiting bodies of Gomphus clavatus Gray was isolated through Sephadex G-200 and DEAE-cellulose columns. The Gomphus clavatus Gray polysaccharide (GCG-1) was mainly composed of β-D-glucosepyranose (β-D-Glu) and α-D-galactopyranose (α-D-Gal) in a ratio of 3:2 and had a molecular weight of ~50,000 Da. The structure of GCG-1 was investigated by a combination of total hydrolysis, gas chromatography-mass spectrometry, methylation analysis, nuclear magnetic resonance spectroscopy and infrared spectra. The results indicated that GCG-1 had a backbone of (1 → 4)-β-D-glucosepyranose residues with branches at O-6 and the branches consisted of two with (1 → 3)-α-D-galactopyranose residue. Antioxidation test in vitro showed that it possessed strong free radical scavenging activity, which may be comparable to vitamin C and butylated hydroxytoluene. GCG-1 also induced the apoptosis of HepG-2 cells and affected the mRNA expression of various housekeeping genes in the HepG-2 cells. The results indicated that Gomphus clavatus Gray may be an ideal sources for antioxidant and anticancer agents. |
| Databáze: | OpenAIRE |
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