De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns
| Autor: | Clara Ferrer Castellà, Céline Cleij, David Foschepoth, Elisa Godino, Anne Doerr, Christophe Danelon, Jonás Noguera López |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Science Lipid Bilayers General Physics and Astronomy Context (language use) Cell Cycle Proteins 010402 general chemistry Biosynthesis 01 natural sciences General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Bacterial Proteins Min System Escherichia coli lcsh:Science Lipid bilayer Cytoskeleton FtsZ Synthetic biology Adenosine Triphosphatases Liposome Multidisciplinary biology Chemistry Escherichia coli Proteins Membrane Proteins General Chemistry Directed evolution 0104 chemical sciences Cell biology Cytoskeletal Proteins 030104 developmental biology Liposomes biology.protein lcsh:Q Artificial Cells FtsA Cell-Free Nucleic Acids Cell Division |
| Zdroj: | Nature Communications Nature Communications, 10(1) Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2041-1723 |
| Popis: | The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Cell-free assays relying on purified proteins have shown that MinE and MinD self-organize into surface waves and oscillatory patterns. In the context of developing a synthetic cell from elementary biological modules, harnessing Min oscillations might allow us to implement higher-order cellular functions. To convey hereditary information, the Min system must be encoded in a DNA molecule that can be copied, transcribed, and translated. Here, the MinD and MinE proteins are synthesized de novo from their genes inside liposomes. Dynamic protein patterns and accompanying liposome shape deformation are observed. When integrated with the cytoskeletal proteins FtsA and FtsZ, the synthetic Min system is able to dynamically regulate FtsZ patterns. By enabling genetic control over Min protein self-organization and membrane remodeling, our methodology offers unique opportunities towards directed evolution of bacterial division processes in vitro. The Min biochemical network regulates bacterial cell division and is a prototypical example of self-organizing molecular systems. Here authors synthesize Min proteins from their genes inside liposomes and observe dynamic protein patterns and liposome shape deformation. |
| Databáze: | OpenAIRE |
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