Effect of biochar-immobilized Sphingomonas sp. PJ2 on bioremediation of PAHs and bacterial community composition in saline soil
| Autor: | Xuguang Niu, Lichao Song, Tianjiao Li, Naiwen Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Environmental Engineering
Soil salinity Health Toxicology and Mutagenesis 0208 environmental biotechnology Polycyclic aromatic hydrocarbon 02 engineering and technology 010501 environmental sciences 01 natural sciences Sphingomonas Soil Bioremediation Dioxygenase Biochar polycyclic compounds Environmental Chemistry Soil Pollutants Polycyclic Aromatic Hydrocarbons Soil Microbiology 0105 earth and related environmental sciences chemistry.chemical_classification biology Chemistry Inoculation Public Health Environmental and Occupational Health General Medicine General Chemistry biology.organism_classification Pollution 020801 environmental engineering Biodegradation Environmental Community composition Environmental chemistry Charcoal Bacteria |
| Zdroj: | Chemosphere. 279 |
| ISSN: | 1879-1298 |
| Popis: | This study aimed to investigate the bioremediation efficiency and bacterial regulation mechanism of biochar-immobilized bacterium (BM) in polycyclic aromatic hydrocarbon (PAH)-contaminated saline soil by conducting pot experiments. In BM treatment, PAH-degrading strain Sphingomonas sp. PJ2 was inoculated into biochar produced at 400 °C and 600 °C using the pine needles (BM400 and BM600). The removal rates of PAHs, soil physicochemical properties, abundance of PAH-ring hydroxylating dioxygenase (PAH-RHD), and bacterial community composition were determined. After 60 days of bioremediation, BM treatment significantly (P 0.05) increased the removal rate of PAHs compared with biochar and PJ2 alone (15.94% and 37.3%, respectively). BM treatment clearly improved the physicochemical properties of saline soil. Moreover, the amount of Gram-positive PAH degraders increased in BM-treated soils compared with other treatments, and their gene abundance had a strong positive correlation with the removal rates of PAHs in soils (r = 0.896; P 0.01). Furthermore, BM treatment increased the abundance of Sphingomonas genus, indicating that the strain PJ2 could survive and colonize in PAH-contaminated saline soil under the protection of biochar. This study provided an effective and green approach for the remediation and improvement of the PAH-contaminated saline soil. |
| Databáze: | OpenAIRE |
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